NumbL is essential for Xenopus primary neurogenesis

BMC Developmental Biology

Kristine A Henningfeld¹  Tomas Pieler¹  Olaf Jahn²  Marie Hedderich¹  Frank Nieber¹ 

[1] Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Goettingen 37077, Germany;Max Planck Institute of Experimental Medicine, Proteomics Group, Hermann-Rein-Str. 3, Goettingen 37075, Germany

关键词: Xenopus;    Neuronal differentiation;    Neurogenin;    Primary neurogenesis;    Notch;    Numb;   

Others  :  1085472
DOI  :  10.1186/1471-213X-13-36

 received in 2013-06-03, accepted in 2013-10-04,  发布年份 2013

【 摘 要 】

Background

Members of the vertebrate Numb family of cell fate determinants serve multiple functions throughout early embryogenesis, including an essential role in the development of the nervous system. The Numb proteins interact with various partner proteins and correspondingly participate in multiple cellular activities, including inhibition of the Notch pathway.

Results

Here, we describe the expression characteristics of Numb and Numblike (NumbL) during Xenopus development and characterize the function of NumbL during primary neurogenesis. NumbL, in contrast to Numb, is expressed in the territories of primary neurogenesis and is positively regulated by the Neurogenin family of proneural transcription factors. Knockdown of NumbL afforded a complete loss of primary neurons and did not lead to an increase in Notch signaling in the open neural plate. Furthermore, we provide evidence that interaction of NumbL with the AP-2 complex is required for NumbL function during primary neurogenesis.

Conclusion

We demonstrate an essential role of NumbL during Xenopus primary neurogenesis and provide evidence for a Notch-independent function of NumbL in this context.

【 授权许可】

   
2013 Nieber et al.; licensee BioMed Central Ltd.

【 图 表 】

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