In vitro antiplasmodial activity of crude extracts of Tetrapleura tetraptera and Copaifera religiosa

BMC Research Notes

Fousseyni S Toure-Ndouo²  Jacques Lebibi³  Rafika Zatra²  Jean Bernard Bongui³  Sandrine Lydie Oyegue Liabagui⁴  Jean Bernard Lekana-Douki¹ 

[1] Unité de Parasitologie Médicale Centre International de Recherches Médicales de Franceville (CIRMF), Département de Parasitologie-Mycologie Médecine Tropicale, Faculté de Médecine Université des Sciences de la Santé. B.P. 769 Franceville Gabon;Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF) B.P. 769 Franceville, Gabon;Unité de Recherches en chimie, Faculté des Sciences, Université des Sciences et Techniques de Masuku, B.P. 943 Franceville, Gabon;Département de Parasitologie-Mycologie Médecine Tropicale, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009 Libreville, Gabon

关键词: Plasmodium falciparum;    cytotoxicity;    Fabaceae, antiplasmodial activity;    Plant extracts;   

Others  :  1166930
DOI  :  10.1186/1756-0500-4-506

 received in 2011-08-16, accepted in 2011-11-23,  发布年份 2011

【 摘 要 】

Background

Malaria remains a major public health problem, especially in tropical and subtropical regions because of the emergence and widespread of antimalarial drug resistance. Traditional medicine represents one potential source of new treatments. Here, we investigated the in vitro antiplasmodial activity of bark extracts from two Fabaceae species (Tetrapleura tertaptera and Copaifera religiosa) traditionally used to treat malaria symptoms in Haut-Ogooué province, Gabon.

Findings

The antiplasmodial activity of dichloromethane and methanolic extracts was tested on P. falciparum strains FCB (chloroquine-resistant) and 3D7 (chloroquine-sensitive) and on fresh clinical isolates, using the DELI method. Host cell toxicity was analyzed on MRC-5 human diploid embryonic lung cells using the MTT test.

The dichloromethane extracts of the two plants had interesting activity (IC₅₀ between 8.5 ± 4.7 and 13.4 ± 3.6 μg/ml). The methanolic extract of Tetrapleura tetraptera was less active (IC₅₀ around 30 μg/ml) and the methanolic extract of Copaifera religiosa was inactive. The selectivity index (toxicity/antiplasmodial activity) of the dichloromethane extract of Tetrapleura tetraptera was high (around 7), while the dichloromethane extract of Copaifera religiosa had the lowest selectivity (0.6). The mean IC₅₀ values for field isolates were less than 1.5 μg/ml for dichloromethane extracts of both plants, while methanolic extracts of Tetrapleura tetraptera showed interesting activity (IC₅₀ = 13.1 μg/ml). The methanolic extract of Copaifera religiosa was also inactive on field isolates.

Conclusions

Dichloromethane extracts of Tetrapleura tetraptera and Copaifera religiosa, two plants used to treat malaria in Gabon, had interesting antiplasmodial activity in vitro. These data provide a scientific rationale for the traditional use of these plants against malaria symptoms. Bioactivity-guided phytochemical analyses are underway to identify the active compounds.

【 授权许可】

   
2011 Lekana-Douki et al; licensee BioMed Central Ltd.

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