Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway in lipopolysaccharide-stimulated RAW 264.7 macrophages

BMC Complementary and Alternative Medicine

Yung Hyun Choi¹  Cheol Park²  Su Hyun Hong⁵  Gi-Young Kim⁵  Min Ho Han¹  Hye Hyeon Lee⁴  Jin-Woo Jeong³ 

[1] Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University, Busan 614-714, Republic of Korea;Department of Molecular Biology, College of Natural Sciences, Dongeui University, Busan 614-714, Republic of Korea;Center for Core Research Facilities, Daegu Gyeongbuk Institute of Science & Technology, Daegu 711-873, Republic of Korea;Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea;Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea

关键词: NF-κB;    Anti-inflammation;    RAW 264.7 cells;    Poria cocos;   

Others  :  1220222
DOI  :  10.1186/1472-6882-14-101

 received in 2013-09-17, accepted in 2014-03-10,  发布年份 2014

【 摘 要 】

Background

Poria cocos Wolf, a medicinal fungus, is widely used in traditional medicines in East Asian countries owing to its various therapeutic potentials. Although several studies have demonstrated the anti-inflammatory activity of this fungus, its underlying mechanisms have not yet been clearly defined.

Methods

In the present study, we have demonstrated the anti-inflammatory effects of ethanol extract of P. cocos (EEPC) in lipopolysaccaride (LPS)-stimulated RAW 264.7 macrophages. As inflammatory parameters, the productions of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin (IL)-1β and tumor necrosis factor (TNF)-α were evaluated. We also examined the EEPC’s effect on the nuclear factor-kappaB (NF-κB) signaling pathway.

Results

Our results indicated that EEPC exhibits a potent inhibitory effect on NO production and inhibits PGE2 release in LPS-induced macrophages without affecting cell viability. EEPC also significantly attenuated LPS-induced secretion of inflammatory cytokines IL-1β and TNF-α. Additionally, LPS-induced expression of inducible NO synthase (iNOS), cyclooxygenase (COX)-2, IL-1β, and TNF-α was decreased by pre-treatment with EEPC at the transcriptional level. Moreover, EEPC clearly inhibited LPS-induced nuclear translocation of NF-κB p65 subunits, which correlated with EEPC’s inhibitory effects on inhibitor kappaB (IκB) degradation. Moreover, EEPC clearly suppressed the LPS-induced DNA-binding activity of NF-κB, as well as the nuclear translocation of the NF-κB p65, which correlated with EEPC’s inhibitory effects on inhibitor kappaB (IκB) degradation.

Conclusions

Taken together, our data indicates that EEPC targets the inflammatory response of macrophages via inhibition of iNOS, COX-2, IL-1β, and TNF-α through inactivation of the NF-κB signaling pathway, supporting the pharmacological basis of P. cocos as a traditional herbal medicine for treatment of inflammation and its associated disorders.

【 授权许可】

   
2014 Jeong et al.; licensee BioMed Central Ltd.

【 图 表 】

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