Anti-inflammatory effect of ethanolic extract from Myagropsis myagroides on murine macrophages and mouse ear edema

BMC Complementary and Alternative Medicine

Research Article

Taisun Shin¹  Bok-Mi Jung¹  Jae-Il Kim²  Hyeung-Rak Kim²  Jong-Soon Kim²  Ji-Woong Choi²  Eun-Ji Joung²  Min-Sup Lee²  Na Young Yoon³  Chi-Won Lim³ 

[1] Department of Food Science and Nutrition, Chonnam National University, 550-749, Yeosu, South Korea;Department of Food Science and Nutrition, Pukyong National University, 608-737, Busan, South Korea;Food and Safety Research Division, National Fisheries Research and Development Institute, 619-705, Gijang-gun, Busan, South Korea;

关键词: Anti-inflammation;    Myagropsis myagroides;    MAPK;    NF-κB;    RAW 264.7 cells;   

DOI  :  10.1186/1472-6882-12-171

 received in 2012-05-21, accepted in 2012-09-27,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThis study aims to investigate anti-inflammatory effect of ethanolic extract of Myagropsis myagroides (EMM) in the lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and the phorbol 12-myristate 13-acetate (PMA)-induced ear edema in mice, and to clarify its underlying molecular mechanisms.MethodsThe levels of nitric oxide (NO), prostaglandin E2 (PGE2), and pro-inflammatory cytokines were measured by Griess assay and enzyme linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinases (MAPKs), and Akt were measured using Western blotting. Nuclear translocation and transcriptional activation of nuclear factor-κB (NF-κB) were determined by immunocytochemistry and reporter gene assay, respectively. PMA-induced mouse ear edema was used as the animal model of inflammation. Anti-inflammatory compounds in EMM were isolated using high-performance liquid chromatography and identified by nuclear magnetic resonance.ResultsEMM significantly inhibited the production of NO, PGE2, and pro-inflammatory cytokines in a dose-dependent manner and suppressed the expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells. EMM strongly suppressed nuclear translocation of NF-κB by preventing degradation of inhibitor of κB-α as well as by inhibiting phosphorylation of Akt and MAPKs. EMM reduced ear edema in PMA-induced mice. One of the anti-inflammatory compounds in EMM was identified as 6,6’-bieckol.ConclusionsThese results suggest that the anti-inflammatory properties of EMM are associated with the down-regulation of iNOS, COX-2, and pro-inflammatory cytokines through the inhibition of NF-κB pathway in LPS-stimulated macrophages.

【 授权许可】

Unknown   
© Joung et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.