Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression

BMC Genomics

Haruko Takeda²  Michel Georges²  Carole Charlier²  Noelle Cockett³  Tracy Hadfield³  Xuewen Xu¹  Huijun Cheng¹ 

[1] Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, P. R. China;Unit of Animal Genomics, GIGA-R & Faculty of Veterinary Medicine, University of Liège (B34), 1 Avenue de l’Hôpital, 4000 Liège, Belgium;Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USA

关键词: Reporter assay;    Post-transcriptional gene regulation;    Trans-effect;    MicroRNA;    DLK1;    Polar overdominance;    Muscle development;    Callipyge;   

Others  :  1127804
DOI  :  10.1186/1471-2164-15-944

 received in 2014-06-15, accepted in 2014-10-16,  发布年份 2014

【 摘 要 】

Background

Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect.

Results

We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo.

Conclusions

This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect.

【 授权许可】

   
2014 Cheng et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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