Taming the wild: resolving the gene pools of non-model Arabidopsis lineages

BMC Evolutionary Biology

Marcus A Koch⁴  Karol Marhold²  Filip Kolář⁵  Magdalena Lučanová⁵  Tzen-Yuh Chiang³  Roswitha Schmickl¹  Nora Hohmann⁴ 

[1] Institute of Botany, Academy of Sciences of the Czech Republic, Průhonice CZ-25243, Czech Republic;Institute of Botany Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava SK-845 23, Slovakia;Department of Life Sciences, Cheng-Kung University, Tainan, Taiwan;Centre for Organismal Studies (COS) Heidelberg, Heidelberg University, Heidelberg 69120, Germany;Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, Prague CZ-128 01, Czech Republic

关键词: Taxonomy;    Systematics;    Microsatellites;    ITS;    Evolution;    Cytology;    Chloroplast;   

Others  :  1117857
DOI  :  10.1186/s12862-014-0224-x

 received in 2014-06-27, accepted in 2014-10-15,  发布年份 2014

【 摘 要 】

Background

Wild relatives in the genus Arabidopsis are recognized as useful model systems to study traits and evolutionary processes in outcrossing species, which are often difficult or even impossible to investigate in the selfing and annual Arabidopsis thaliana. However, Arabidopsis as a genus is littered with sub-species and ecotypes which make realizing the potential of these non-model Arabidopsis lineages problematic. There are relatively few evolutionary studies which comprehensively characterize the gene pools across all of the Arabidopsis supra-groups and hypothesized evolutionary lineages and none include sampling at a world-wide scale. Here we explore the gene pools of these various taxa using various molecular markers and cytological analyses.

Results

Based on ITS, microsatellite, chloroplast and nuclear DNA content data we demonstrate the presence of three major evolutionary groups broadly characterized as A. lyrata group, A. halleri group and A. arenosa group. All are composed of further species and sub-species forming larger aggregates. Depending on the resolution of the marker, a few closely related taxa such as A. pedemontana, A. cebennensis and A. croatica are also clearly distinct evolutionary lineages. ITS sequences and a population-based screen based on microsatellites were highly concordant. The major gene pools identified by ITS sequences were also significantly differentiated by their homoploid nuclear DNA content estimated by flow cytometry. The chloroplast genome provided less resolution than the nuclear data, and it remains unclear whether the extensive haplotype sharing apparent between taxa results from gene flow or incomplete lineage sorting in this relatively young group of species with Pleistocene origins.

Conclusions

Our study provides a comprehensive overview of the genetic variation within and among the various taxa of the genus Arabidopsis. The resolved gene pools and evolutionary lineages will set the framework for future comparative studies on genetic diversity. Extensive population-based phylogeographic studies will also be required, however, in particular for A. arenosa and their affiliated taxa and cytotypes.

【 授权许可】

   
2014 Hohmann et al.; licensee BioMed Central Ltd.

【 图 表 】

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