期刊论文详细信息
BMC Infectious Diseases
Performance comparison of phenotypic and molecular methods for detection and differentiation of Candida albicans and Candida dubliniensis
Rachel Chandy1  Ajmal Theyyathel1  Mohammad Asadzadeh1  Ziauddin Khan1  Suhail Ahmad1 
[1] Department of Microbiology, Faculty of Medicine, Kuwait University, P. O. Box 24923, Safat, 13110, Kuwait
关键词: Duplex PCR;    Differentiation;    Detection;    Candida dubliniensis;    Candida albicans;   
Others  :  1159661
DOI  :  10.1186/1471-2334-12-230
 received in 2012-06-10, accepted in 2012-09-24,  发布年份 2012
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【 摘 要 】

Background

Candida albicans is the most pathogenic Candida species but shares many phenotypic features with Candida dubliniensis and may, therefore, be misidentified in clinical microbiology laboratories. Candidemia cases due to C. dubliniensis are increasingly being reported in recent years. Accurate identification is warranted since mortality rates are highest for C. albicans infections, however, C. dubliniensis has the propensity to develop resistance against azoles more easily. We developed a duplex PCR assay for rapid detection and differentiation of C. albicans from C. dubliniensis for resource-poor settings equipped with basic PCR technology and compared its performance with three phenotypic methods.

Methods

Duplex PCR was performed on 122 germ tube positive and 12 germ tube negative isolates of Candida species previously identified by assimilation profiles on Vitek 2 ID-YST system. Typical morphologic characteristics on simplified sunflower seed agar (SSA), and reaction with a commercial (Bichro-Dubli) latex agglutination test were also performed. The assay was further applied on 239 clinical yeast and yeast-like fungi and results were confirmed by DNA sequencing of internal transcribed spacer (ITS) region of rDNA.

Results

The results of duplex PCR assay for 122 germ tube positive and 12 germ tube negative isolates of Candida species were comparable to their identification by Vitek 2 ID-YST system, colony characteristics on SSA and latex agglutination test. Application of duplex PCR also correctly identified all 148 C. albicans and 50 C. dubliniensis strains among 239 yeast-like fungi.

Conclusions

The data show that both, duplex PCR and Bichro-Dubli are reliable tests for rapid (within few hours) identification of clinical yeast isolates as C. dubliniensis or C. albicans. However, duplex PCR may be applied directly on clinical yeast isolates for their identification as C. dubliniensis or C. albicans as it does not require prior testing for germ tube formation or latex Candida agglutination.

【 授权许可】

   
2012 Ahmad et al.; licensee BioMed Central Ltd.

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