In vitro biological action of aqueous extract from roots of Physalis angulata against Leishmania (Leishmania) amazonensis

BMC Complementary and Alternative Medicine

Edilene O. Silva²  José Luiz M. do Nascimento³  Gilmara N. T. Bastos³  Danila Teresa V. Alves⁴  Milton N. da Silva⁴  Luis Henrique S. Farias¹  Ana Paula D. Rodrigues⁵  Bruno J. M. da Silva¹  Raquel Raick P. da Silva¹ 

[1] Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagem, Ilha do Fundão, Rio de Janeiro, 21941 902, Rio de Janeiro, Brazil;Universidade Federal do Pará, Laboratório de Biologia Estrutural, Avenida Augusto Corrêa, 01, Bairro Guamá, Belém, 660975-110, Pará, Brazil;Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Neuroquímica Molecular e Celular, Avenida Augusto Corrêa, 01, Bairro Guamá, Belém, 660975-110, Pará, Brazil;Universidade Federal do Pará, Instituto de Ciências Exatas e Naturais, Laboratório de Cromatografia Líquida, Avenida Augusto Corrêa, 01, Bairro Guamá, Belém, 660975-110, Pará, Brazil;Instituto Evandro Chagas, Laboratório de Microscopia Eletrônica, SVS, MS, Av. Almirante Barroso 492, Bairro Marco, Belém, 66090-000, Pará, Brazil

关键词: Antileishmanial activity;    Macrophage viability;    Morphological alterations;    Leishmania amazonensis;    Physalins;    Aqueous extract;    Physalis angulata;   

Others  :  1221179
DOI  :  10.1186/s12906-015-0717-1

 received in 2014-10-23, accepted in 2015-06-11,  发布年份 2015

【 摘 要 】

Background

Leishmaniasis is an infectious disease caused by various species of the protozoan parasites of the Leishmania genus and transmitted by phlebotomine sandflies. The protozoa multiply in phagocytic cells, mainly macrophages, which play an important role defending the organism from pathogens. The most effective treatment for leishmaniasis is the chemotherapy and besides the high cost, these drugs are toxic and require a long period of treatment. Currently, some herbal products are considered an important alternative source of a new leishmanicidal agent, which includes the plant Physalis angulata, . We evaluated effects of an aqueous extract from roots of Physalis angulata (AEPa) on Leishmania proliferation, morphology and also determined whether physalins were present in the extract contributing to the knowledge of its pharmacological efficacy.

Methods

Morphological alterations were determined by light microscopy, transmission and scanning electron microscopy. Host cell viability was evaluated by MTT, and propidium iodide. AEPa were submitted in full HRESITOF analysis.

Results

AEPa promoted a dose-dependent reduction on promastigotes (IC ₅₀  = 39.5 μg/mL ± 5.1) and amastigotes (IC ₅₀  = 43.4 μg/mL ± 10.1) growth. This growth inhibition was associated with several morphological alterations observed in promastigote forms. No cytotoxic effect in mammalian cells was detected (IC ₅₀  > 4000 μg/mL). Furthemore, the presence of physalins A, B, D, E, F, G and H were described, for the first time, in the P. angulata root.

Conclusions

Results demonstrate that AEPa effectively promotes antileishmanial activity with several important morphological alterations and has no cytotoxic effects on host cells.

【 授权许可】

   
2015 da Silva et al.

【 图 表 】

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