Crystal structure of putative CbiT from Methanocaldococcus jannaschii: an intermediate enzyme activity in cobalamin (vitamin B12) biosynthesis

BMC Structural Biology

Yoshitaka Bessho¹  Shigeyuki Yokoyama²  Balasundaram Padmanabhan³ 

[1] RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan;Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan;RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

关键词: Methanocaldococcus jannaschii;    MJ0391;    AdoHcy cofactor;    Crystal structure;    CbiT;    Decarboxylase;    Precorrin 7 C15-methyltransferase;    Vitamin B₁₂;    Cobalamin biosynthesis;   

Others  :  1091279
DOI  :  10.1186/1472-6807-13-10

 received in 2013-02-02, accepted in 2013-05-10,  发布年份 2013

【 摘 要 】

Background

In the anaerobic pathway of cobalamin (vitamin B₁₂) synthesis, the CbiT enzyme plays two roles, as a cobalt-precorrin-7 C15-methyltransferase and a C12-decarboxylase, to produce the intermediate, cobalt-precorrin 8.

Results

The primary structure of the hypothetical protein MJ0391, from Methanocaldococcus jannaschii, suggested that MJ0391 is a putative CbiT. Here, we report the crystal structure of MJ0391, solved by the MAD procedure and refined to final R-factor and R-free values of 19.8 & 27.3%, respectively, at 2.3 Å resolution. The asymmetric unit contains two NCS molecules, and the intact tetramer generated by crystallographic symmetry may be functionally important. The overall tertiary structure and the tetrameric arrangements are highly homologous to those found in MT0146/CbiT from Methanobacterium thermoautotrophicum.

Conclusions

The conservation of functional residues in the binding site for the co-factor, AdoMet, and in the putative precorrin-7 binding pocket suggested that MJ0391 may also possess CbiT activity. The putative function of MJ0391 is discussed, based on structural homology.

【 授权许可】

   
2013 Padmanabhan et al.; licensee BioMed Central Ltd.

【 图 表 】

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