CsrA impacts survival of Yersinia enterocolitica by affecting a myriad of physiological activities

BMC Microbiology

Glenn M Young¹  Jing-Yu Chen³  Gulustan Ozturk¹  Kang K Liu¹  Yan Zheng²  Shane Petersen¹  Karen LeGrand¹ 

[1] Department of Food Science and Technology, University of California, Davis, Davis, CA, USA;College of Food Science, Shenyang Agricultural University, Shenyang, PR China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China

关键词: Mutant selection;    Psychrotroph;    Temperature sensitivity;    Antibiotic sensitivity;    Salt sensitivity;    Motility;    Csr system;    CsrA;    Yersinia;   

Others  :  1137431
DOI  :  10.1186/s12866-015-0343-6

 received in 2014-10-01, accepted in 2015-01-13,  发布年份 2015

【 摘 要 】

Background

A previous study identified a Yersinia enterocolitica transposon mutant, GY448, that was unable to export the flagellar type three secretion system (T3SS)-dependent phospholipase, YplA. This strain was also deficient for motility and unable to form colonies on Lauria-Bertani agar medium. Preliminary analysis suggested it carried a mutation in csrA. CsrA in Escherichia coli is an RNA-binding protein that is involved in specific post-transcriptional regulation of a myriad of physiological activities. This study investigated how CsrA affects expression of the flagellar regulatory cascade that controls YplA export and motility. It also explored the effect of csrA mutation on Y. enterocolitica in response to conditions that cue physiological changes important for growth in environments found both in nature and the laboratory.

Results

The precise location of the transposon insertion in GMY448 was mapped within csrA. Genetic complementation restored disruptions in motility and the YplA export phenotype (Yex), which confirmed this mutation disrupted CsrA function. Mutation of csrA affected expression of yplA and flagellar genes involved in flagellar T3SS dependent export and motility by altering expression of the master regulators flhDC. Mutation of csrA also resulted in increased sensitivity of Y. enterocolitica to various osmolytes, temperatures and antibiotics.

Conclusions

The results of this study reveal unique aspects of how CsrA functions in Y. enterocolitica to control its physiology. This provides perspective on how the Csr system is susceptible to adaptation to particular environments and bacterial lifestyles.

【 授权许可】

   
2015 LeGrand et al.; licensee BioMed Central.

【 图 表 】

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