Comparison among nasopharyngeal swab, nasal wash, and oropharyngeal swab for respiratory virus detection in adults with acute pharyngitis

BMC Infectious Diseases

Nan-Shan Zhong²  Zi-Feng Yang²  Yan-Fang Wang³  Yun-Ying Li¹  Qiao-Yan Chen¹  Li Li² 

[1] Guangdong Provincial Hospital of Chinese Medicine, The Second Clinical College of Guangzhou University of C.M., Guangdong Provincial Academy of Chinese Medical Sciences, 111 Da De Road, Guangzhou 510120, China;Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Diseases (Guangzhou Medical University), The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, Guangzhou 510120, China;South China Normal University, 55 Zhong Shan Road West, Guangzhou 510631, China

关键词: TaqMan real-time polymerase chain reaction;    Sensitivity;    Nasal wash;    Nasopharyngeal swab;    Oropharyngeal swab;    Acute pharyngitis;    Respiratory viruses;   

Others  :  1147724
DOI  :  10.1186/1471-2334-13-281

 received in 2013-01-13, accepted in 2013-06-17,  发布年份 2013

【 摘 要 】

Background

Acute pharyngitis is frequently seen in primary care. Acute viral pharyngitis may be easily misdiagnosed as acute bacterial pharyngitis. Laboratory-confirmed diagnosis of respiratory viruses is recommended. The purpose of this study was to compare the sensitivities among oropharyngeal swab (OPS), nasopharyngeal swab (NPS), and nasal wash (NW) in adults with acute pharyngitis.

Methods

OPS, NPS, and NW were obtained from each participant with acute pharyngitis. The specimens were tested for 15 respiratory viruses by TaqMan real-time polymerase chain reaction. A sample was considered to be a true positive if any of the specimens was positive. The sensitivities among samples were compared by chi-square test or Fisher’s exact test, as appropriate.

Results

One hundred three triple samples collected consecutively by OPS, NPS, and NW were obtained. In 73 patients, one or more viruses were detected by any of the three methods. Among all viruses, the sensitivity of NPS was significantly higher than that of NW (74% vs. 49%, respectively; p < 0.01) and OPS (74% vs. 49%, respectively; p < 0.01).

Conclusions

Flocked NPS collection may be the most effective alternative to NW and OPS for detection of respiratory viruses in adults with acute pharyngitis using TaqMan real-time polymerase chain reaction.

【 授权许可】

   
2013 Li et al.; licensee BioMed Central Ltd.

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