Immune responses in the lungs of patients with tuberculous pleural effusion without pulmonary tuberculosis

BMC Immunology

Kun Young Kwon³  Won-Il Choi²  Diana Qama¹ 

[1] Regional Hospital, Berat, Albania;Department of Internal Medicine, Dongsan Hospital, Keimyung University School of Medicine, Dalseong-ro 56, Jung-gu, Daegu, 700-712, Republic of Korea;Department of Pathology, Dongsan Hospital, Keimyung University School of Medicine, Dalseong-ro 56, Jung-gu, Daegu, 700-712, Republic of Korea

关键词: Vascular endothelial growth factor;    Tumor necrosis factor-α;    Tuberculosis;    Interferon-γ;   

Others  :  1077886
DOI  :  10.1186/1471-2172-13-45

 received in 2012-04-06, accepted in 2012-07-23,  发布年份 2012

【 摘 要 】

Background

Tuberculous pleural effusion (TPE) is one of the most common forms of extrapulmonary tuberculosis. Because most studies of TPE focused on the pleural space, little information regarding lung parenchyma is available. We therefore aimed to investigate immune responses in the lung parenchyma of TPE patients without pulmonary tuberculosis.

Methods

Patients with any evidence of pulmonary tuberculosis, either from radiologic or bacteriologic evaluation, were excluded. Bronchoalveolar lavage fluid (BALF) was collected from 10 newly diagnosed, untreated, HIV-negative TPE patients and 10 healthy controls. We analyzed T-lymphocyte subpopulations and measured 10 cytokines in BALF. Cytokine levels in BALF were standardised using urea.

Results

The concentrations of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF), and the CD4+/CD8+ ratio of T-lymphocytes were significantly higher in TPE patients without pulmonary tuberculosis than in the controls. Of the cytokines measured in BALF, VEGF showed the highest concentration. No difference was observed in T-helper type 2 cytokines between the 2 groups.

Conclusion

There were significant immune responses and increases in IFN-γ, TNF-α, and VEGF in the lung parenchyma of TPE patients without pulmonary tuberculosis. This result suggests that TPE may induce a significant immune response in lung parenchyma.

【 授权许可】

   
2012 Qama et al.; licensee BioMed Central Ltd.

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