Changes in activity of metabolic and regulatory pathways during germination of S. coelicolor

BMC Genomics

Jiri Vohradsky¹  Alice Zikova¹  Eva Strakova¹  Jan Bobek² 

[1] Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Bioinformatics, Vídeňská 1083, 142 20 Prague 4, Czech Republic;Chemistry Department, Faculty of Science, J. E. Purkinje University, 400 96 Ústí nad Labem, Czech Republic

关键词: Metabolic pathways;    Sigma factors;    Microarrays;    Streptomyces;    Germination;   

Others  :  1121481
DOI  :  10.1186/1471-2164-15-1173

 received in 2014-07-08, accepted in 2014-12-15,  发布年份 2014

【 摘 要 】

Background

Bacterial spore germination is a developmental process during which all required metabolic pathways are restored to transfer cells from their dormant state into vegetative growth. Streptomyces are soil dwelling filamentous bacteria with complex life cycle, studied mostly for they ability to synthesize secondary metabolites including antibiotics.

Results

Here, we present a systematic approach that analyzes gene expression data obtained from 13 time points taken over 5.5 h of Streptomyces germination. Genes whose expression was significantly enhanced/diminished during the time-course were identified, and classified to metabolic and regulatory pathways. The classification into metabolic pathways revealed timing of the activation of specific pathways during the course of germination. The analysis also identified remarkable changes in the expression of specific sigma factors over the course of germination. Based on our knowledge of the targets of these factors, we speculate on their possible roles during germination. Among the factors whose expression was enhanced during the initial part of germination, SigE is though to manage cell wall reconstruction, SigR controls protein re-aggregation, and others (SigH, SigB, SigI, SigJ) control osmotic and oxidative stress responses.

Conclusions

From the results, we conclude that most of the metabolic pathway mRNAs required for the initial phases of germination were synthesized during the sporulation process and stably conserved in the spore. After rehydration in growth medium, the stored mRNAs are being degraded and resynthesized during first hour. From the analysis of sigma factors we conclude that conditions favoring germination evoke stress-like cell responses.

【 授权许可】

   
2015 Bobek et al.; licensee BioMed Central.

【 图 表 】

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