Lutein Dietary Supplementation Attenuates Streptozotocin-induced testicular damage and oxidative stress in diabetic rats

BMC Complementary and Alternative Medicine

Mohammed M. Ahmed²  Mihir Y. Parmar²  Abdullah Al-Assaf¹  Hatem M. Abuohashish³  Salim S. Al-Rejaie²  Amal J. Fatani² 

[1] Department of Science and Nutrition, College of Food and Agricultural Sciences, King Saud University-11544, Riyadh, Saudi Arabia;Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11544, Saudi Arabia;Department of Biomedical Dental Sciences, College of Dentistry, University of Dammam, Dammam-31441, Saudi Arabia

关键词: Testicular cells;    Oxidative stress;    Diabetes mellitus;    Lutein;   

Others  :  1216994
DOI  :  10.1186/s12906-015-0693-5

 received in 2014-12-25, accepted in 2015-05-26,  发布年份 2015

【 摘 要 】

Background

Diabetes mellitus with the successive generation of reactive oxygen species signifies a major risk factor for testicular dysfunction. Antioxidant supplements are one of the best options to prevent such disorder. In the present study, lutein as dietary supplement has been used to explore its potential protective effects against diabetes-induced oxidative stress in testicular cells.

Methods

Diabetes was induced using a single IP injection of streptozotocin (STZ). Lutein was mixed with rat chow powder and supplemented to diabetic rats for 5 weeks. Serum testosterone levels were estimated. In testicular cells, thiobarbituric acid reactive substances (TBARS), total sulfhydryl groups (T-GSH), non-protein sulfhydryl groups (NP-SH), superoxide dismutase (SOD) and catalase (CAT) activities were measured. Pro-inflammatory mediators like tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) were measured in the testis. Nucleic acids and total protein (TP) levels were also estimated in testicular cells. Histopathological changes were evaluated in testis.

Results

Serum testosterone level was significantly decreased in diabetic animals compared to controls. Diabetes markedly reduced T-GSH, NP-SH, CAT and SOD, while TBARS, TNF-α and IL-1β levels were increased in the diabetic testis compared to non-diabetic controls. Lutein supplementation, significantly and dose dependently increased the serum testosterone level. The elevated TBARS levels were significantly decreased compared to diabetic group, while the decreased levels of T-GSH and NP-SH and activities of CAT and SOD were found increased by lutein treatments in dose dependent manner. Lutein pretreatment also inhibited the TNF-α and IL-1β levels compared to diabetic group. The decreased values of nucleic acids and total protein in diabetic group were also significantly increased in lutein supplemented groups. The histopathological evaluation revealed protection the damaged testicular cells in the diabetic rats by lutein supplementation.

Conclusion

These findings showed that lutein has potential beneficial effects in diabetes-induced testicular damage, probably through its antioxidant and anti-inflammatory properties.

【 授权许可】

   
2015 Fatani et al.

【 图 表 】

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