Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling

BMC Musculoskeletal Disorders

Frank Buttgereit⁴  Hong Zhou⁵  Markus J Seibel²  Dörte Huscher³  Timo Gaber¹  Aiqing Li⁵  Jinwen Tu⁵  Edgar Wiebe⁵  Cornelia M Spies⁵ 

[1] Berlin-Brandenburg Centre of Regenerative Therapies (BCRT), Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany;Department of Endocrinology & Metabolism, Concord Repatriation Hospital, Sydney, Australia;German Rheumatism Research Centre (DRFZ), Charitéplatz 1, D-10117 Berlin, Germany;Department of Rheumatology and Clinical Immunology, Charité – Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany;Bone Research Program, ANZAC Research Institute, Concord Repatriation Hospital, The University of Sydney, Hospital Road, Concord Sydney, NSW 2139, Australia

关键词: Osteoblasts;    Glucocorticoids;    Arthritis;    Animal;    Disease models;    11-beta-hydroxysteroid dehydrogenase type 2;   

Others  :  1128854
DOI  :  10.1186/1471-2474-15-31

 received in 2013-12-31, accepted in 2014-01-29,  发布年份 2014

【 摘 要 】

Background

The role of endogenous glucocorticoids (GC) in the initiation and maintenance of rheumatoid arthritis (RA) remains unclear. We demonstrated previously that disruption of GC signalling in osteoblasts results in a profound attenuation of K/BxN serum-induced arthritis, a mouse model of RA. To determine whether or not the modulation of the inflammatory response by osteoblasts involves T cells, we studied the effects of disrupted osteoblastic GC-signalling in the T cell-dependent model of antigen-induced arthritis (AIA).

Methods

Acute arthritis was induced in pre-immunised 11-week-old male 11β-hydroxysteroid dehydrogenase type 2 transgenic (tg) mice and their wild-type (WT) littermates by intra-articular injection of methylated bovine serum albumine (mBSA) into one knee joint. Knee diameter was measured every 1–2 days until euthanasia on day 14 post injection. In a separate experiment, arthritis was maintained for 28 days by weekly reinjections of mBSA. Tissues were analysed by histology, histomorphometry and microfocal-computed tomography. Serum cytokines levels were determined by multiplex suspension array.

Results

In both short and long term experiments, arthritis developed in tg and WT mice with no significant difference between both groups. Histological indices of inflammation, cartilage damage and bone erosion were similar in tg and WT mice. Bone volume and turnover at the contralateral tibia and systemic cytokine levels were not different.

Conclusions

Acute murine AIA is not affected by a disruption in osteoblastic GC signalling. These data indicate that osteoblasts do not modulate the T cell-mediated inflammatory response via a GC-dependent pathway.

【 授权许可】

   
2014 Spies et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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