Si-Wu-tang extract stimulates bone formation through PI3K/Akt/NF-κB signaling pathways in osteoblasts

BMC Complementary and Alternative Medicine

Chih-Hsin Tang³  Yi-Chin Fong⁴  Te-Mao Li²  Po-Chun Chen¹  Chi-Ming Wu² 

[1] Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan;School of Chinese Medicine, China Medical University, Taichung, Taiwan;Department of Biotechnology, College of Health Science, Asia University, Taichung, Taiwan;Department of Orthopaedics, China Medical University Hospital, Taichung, Taiwan

关键词: Traditional Chinese medicine;    Bone formation;    Osteoblasts;    SWT;   

Others  :  1220883
DOI  :  10.1186/1472-6882-13-277

 received in 2013-06-18, accepted in 2013-10-17,  发布年份 2013

【 摘 要 】

Background

Si-Wu-Tang (SWT), a Traditional Chinese Medicine (TCM) formula, is widely used for the treatment of gynopathies diseases such as menstrual discomfort, climacteric syndrome, dysmenorrhea, and other estrogen-related diseases. Recent studies have shown that SWT can treat primary dysmenorrhea, have anti-pruritic anti-inflammatory effects, and protect against radiation-induced bone marrow damage in an animal model. It has been reported that anti-inflammatory and anti-oxidant agents have the potential to treat osteoporosis by increasing bone formation and/or suppressing bone resorption. However, the effect of SWT on bone cell function has not yet been reported.

Methods

Alkaline phosphatase (ALP), bone morphogenetic proteins (BMP)-2, and osteopontin (OPN) mRNA expression was analyzed by qPCR. The mechanism of action of SWT extract was investigated using western blotting. The in vivo anti-osteoporotic effect of SWT extract was assessed in ovariectomized mice.

Results

Here, we report that SWT increases ALP, BMP-2, and OPN expression as well as bone mineralization. In addition, we show that the PI3K, Akt, and NF-κB signaling pathways may be involved in the SWT-mediated increase in gene expression and bone mineralization. Notably, treatment of mice with SWT extract prevented bone loss induced by ovariectomy in vivo.

Conclusion

SWT may be used to stimulate bone formation for the treatment of osteoporosis.

【 授权许可】

   
2013 Wu et al.; licensee BioMed Central Ltd.

【 图 表 】

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