Effects of dimethyloxalylglycine on wound healing of palatal mucosa in a rat model

BMC Oral Health

Hyeong-Cheol Yang¹  Kyung Mi Son¹  Hee Chul Park¹  Tingting Zhu¹ 

[1] Department of Dental Biomaterials Science and Dental Research Institute, College of Dentistry, Seoul National University, 28 Yeonkun-dong, Chongro-ku 110-749, Seoul, South Korea

关键词: Wound healing;    Palatal mucosa;    Vascular endothelial growth factor;    Hypoxia-inducible factor 1 alpha;    Dimethyloxalylglycine;   

Others  :  1203900
DOI  :  10.1186/s12903-015-0047-1

 received in 2015-02-04, accepted in 2015-05-13,  发布年份 2015

【 摘 要 】

Background

Rapid wound healing of oral soft tissue may reduce the opportunity of infection and discomfort of patients. Previous studies have demonstrated that enhancement of angiogenesis is an effective way to accelerate wound repair. In this study, to enhance angiogenesis and healing of palatal wounds, dimethyloxalylglycine (DMOG) was applied to a rat palatal wound model. DMOG is known to inhibit oxygen-dependent degradation of hypoxia inducible factor-1 alpha (HIF-1α), which can lead to up-regulation of angiogenesis markers, favoring wound repair. We also evaluated the effects of DMOG on cell migration and HIF-1α expression of rat palatal (RP) cells. Furthermore, mRNA and protein expression of vascular endothelial growth factor (VEGF) were analyzed in DMOG-treated RP cells.

Methods

Primary cultures of rat palatal (RP) cells were obtained from Sprague–Dawley (SD) rats. Effects of DMOG on cell viability and migration of RP cells were evaluated by using a formazan and culture insert, respectively. VEGF mRNA was observed by real-time PCR, and VEGF and HIF-1α proteins were detected by Western blotting. For the animal study, excisional wounds, 3 mm in diameter, were made at the central part of the palate of SD rats. DMOG with hyaluronic acid ointment was topically applied three times during 1 week, and then wound closures were quantitated photographically and histologically.

Results

DMOG was cytotoxic to RP cells at concentrations higher than 2 mM and did not affect cell migration at non-cytotoxic concentrations. mRNA and protein expression of VEGF were significantly stimulated by DMOG treatment. The protein level of HIF-1α was also stabilized in RP cells by DMOG. In the animal study, groups treated with 1 mg/ml DMOG showed an increase of rat palatal wound contractures.

Conclusions

DMOG enhanced wound healing of rat palatal mucosa, which was likely due to the angiogenic effect of the agent.

【 授权许可】

   
2015 Zhu et al.; licensee BioMed Central.

【 图 表 】

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