Clinical and pathological features of Nerium oleander extract toxicosis in wistar rats

BMC Research Notes

Muddasir Hassan Abbasi²  Nadeem Sheikh¹  Tasleem Akhtar¹ 

[1] Cell and Molecular Biology Lab, Department of Zoology, University of the Punjab, Q-A Campus, Lahore 54590, Pakistan;Department of Zoology, Government College of Science, Wahdat Road, Lahore 54590, Pakistan

关键词: Neutrophilia;    Macrocytosis;    Polycythemia;    Toxicity;    Inflammation;    Histopathology;   

Others  :  1091081
DOI  :  10.1186/1756-0500-7-947

 received in 2014-04-28, accepted in 2014-12-12,  发布年份 2014

【 摘 要 】

Background

Nerium oleander has been widely studied for medicinal purposes for variety of maladies. N. oleander has also been reported having noxious effects because of its number of components that may show signs of toxicity by inhibiting plasma lemma Na⁺, K⁺-ATPase. The present study was performed to scrutinize the toxic effect of N. oleander leaves extract and its clinical and pathological features in wistar rats.

Results

Hematological analysis showed significant variations in RBCs count (P = 0.01), Hb (P = 0.001), Hct (P = 0.0003), MCV (P = 0.013), lymphocyte count (P = 0.015), neutrophil count (P = 0.003), monocyte count (P = 0.012) and eosinophil count (P = 0.006). Histopathological studies have shown that in T₁ group noticeable infiltration of inflammatory cells was found with low level of vascular damage. In T₂ group, increased proportion of binucleated and inflammatory cells, hepatic necrosis, widening of sinusoidal spaces and mild level of vascular damage was observed.

Conclusion

Taken together these findings we can conclude that N. oleander leaves extract significantly affects on experimental animals due to its toxicity. Efforts must be exerted to purify different chemical components from extract with no inflammation as this plant is utilized in folk medicine with narrow therapeutic indices.

【 授权许可】

   
2014 Akhtar et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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