Diet-induced variability of the resistin gene (Retn) transcript level and methylation profile in rats

BMC Genetics

Izabela Szczerbal¹  Slawomir Sadkowski¹  Maciej Szydlowski¹  Ewa Pruszynska-Oszmalek²  Joanna Nowacka-Woszuk¹ 

[1] Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wolynska 33, Poznan, 60-637, Poland;Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Wolynska 35, Poznan 60-637, Poland

关键词: DNA methylation;    Gene expression;    Diets;    Obesity;    Rat;    Resistin;   

Others  :  1225208
DOI  :  10.1186/s12863-015-0270-4

 received in 2015-06-05, accepted in 2015-09-10,  发布年份 2015

【 摘 要 】

Background

Adipose tissue is recognized as a highly active metabolic and endocrine organ. The hormones secreted by this tissue play an important role in many biochemical processes. It is known that dysfunction of adipocytes can cause insulin resistance, type 2 diabetes or hyperlipidemia. One of the important factors produced in fat tissue is resistin (Retn). It has been postulated that this hormone is involved in glucose homeostasis and insulin resistance. In the present study, the impact of five diet types (ad libitum normal, restricted, high-carbohydrate, high-fat and high-protein) on the Retn gene transcription and methylation profile was evaluated in rats of different ages.

Results

Transcript levels and methylation status of the Retn gene were studied in three tissues (muscle, subcutaneous and abdominal fat) in rats at 30, 60 and 120 days of age. We found an effect of tissue type on the Retn transcription in all diet types, as well as an effect of feeding type and age on the mRNA levels for high-fat and high-protein diets. The DNA methylation levels depended only on tissue type.

Conclusions

The obtained results demonstrate a tissue-specific expression pattern and a characteristic DNA methylation profile of the Retn gene in rats. Retn expression seems to be sensitive to nutritional changes, but only in the case of high-fat and high-protein diets. Moreover, an effect of age on Retn mRNA content was observed in these diets. Because no correlation between the transcript level and methylation status was found, we assumed that the transcription control of this gene by DNA methylation of the promoter seems to be unlikely.

【 授权许可】

   
2015 Nowacka-Woszuk et al.

【 图 表 】

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