Antinociceptive and anti-tussive activities of the ethanol extract of the flowers of Meconopsis punicea Maxim

BMC Complementary and Alternative Medicine

Hu Pan¹  Yu Zhang²  Xuezhi Wang¹  Jiyu Zhang¹  Yu Wang¹  Xiaolou Miao¹  Dongsheng Wang¹  Xiaofei Shang¹ 

[1] Key Laboratory of New Animal Drug Project, Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science, Lanzhou 730050, People’s Republic of China;Department of Emergency, Lanzhou General Hospital of PLA, Lanzhou 730050, People’s Republic of China

关键词: Antitussive activity;    Formalin test;    Antinociceptive activity;    Meconopsis punicea extract;   

Others  :  1205852
DOI  :  10.1186/s12906-015-0671-y

 received in 2014-12-12, accepted in 2015-05-13,  发布年份 2015

【 摘 要 】

Background

As an important traditional Tibetan (veterinary) medicine, the flowers of Meconopsis punicea (family Papaveraceae) have been used to treat pain, fever, cough, inflammation, liver heat and lung heat of humans and animals by local people for thousands of years. In this paper, we aimed to investigate the antinociceptive and anti-tussive activities of the ethanol extract of M. punicea (EEM).

Methods

Firstly, HPLC was used to analyze the main constituents of the ethanol extract of M. punicea. In animal experiments, the acetic acid-induced writhing response test, hot plate test, barbiturate-induced sleeping time and formalin tests were used to evaluate the antinociceptive activity. Then, ammonia-induced coughing and sulfur dioxide-induced coughing tests in mice as well as the phenol red secretion in trachea test were used to investigate the anti-tussive activity of the extract. Finally, an acute toxicity study was carried out.

Results

The results showed that alkaloids and flavonoids were the main compounds in the ethanol extract of M. punicea flowers. The extract at 125, 250 and 500 mg/kg had good antinociceptive and anti-tussive activities in mice with a dose-dependent manner.

Conclusions

These findings suggested that EEM has significant bioactivities, and the active components of M. punicea should be studied further.

【 授权许可】

   
2015 Shang et al.; licensee BioMed Central.

【 图 表 】

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