Measuring telomere length for the early detection of precursor lesions of esophageal squamous cell carcinoma

BMC Cancer

Shih-Wen Lin¹  Christian C Abnet¹  Neal D Freedman¹  Gwen Murphy¹  Rosana Risques³  Donna Prunkard³  Peter Rabinovitch³  Qin-Jing Pan⁴  Mark J Roth²  Guo-Qing Wang⁴  Wen-Qiang Wei⁴  Ning Lu⁴  Philip R Taylor¹  You-Lin Qiao⁴  Sanford M Dawsey¹ 

[1] Division of Cancer Epidemiology & Genetics, National Cancer Institute, 9609 Medical Center Drive, Bethesda, MD 20892, USA

[2] Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Building 10, Bethesda, MD 20892, USA

[3] Department of Pathology, University of Washington, 1959 NE Pacific Ave., Seattle, WA 98195, USA

[4] Cancer Institute, Chinese Academy of Medical Sciences, P. O. Box 2258, Beijing 100021, People’s Republic of China

关键词: Telomeres;    Balloon cytology;    Screening;    Early detection;    Esophageal squamous dysplasia;    Esophageal squamous cell carcinoma;   

Others  :  859220
DOI  :  10.1186/1471-2407-13-578

 received in 2013-10-09, accepted in 2013-11-27,  发布年份 2013

【 摘 要 】

Background

Esophageal cancer is the sixth leading cause of cancer death worldwide; current early detection screening tests are inadequate. Esophageal balloon cytology successfully retrieves exfoliated and scraped superficial esophageal epithelial cells, but cytologic reading of these cells has poor sensitivity and specificity for detecting esophageal squamous dysplasia (ESD), the precursor lesion of esophageal squamous cell carcinoma (ESCC). Measuring telomere length, a marker for chromosomal instability, may improve the utility of balloon cytology for detecting ESD and early ESCC.

Methods

We examined balloon cytology specimens from 89 asymptomatic cases of ESD (37 low-grade and 52 high-grade) and 92 age- and sex-matched normal controls from an esophageal cancer early detection screening study. All subjects also underwent endoscopy and biopsy, and ESD was diagnosed histopathologically. DNA was extracted from the balloon cytology cells, and telomere length was measured by quantitative PCR. A receiver operating characteristic (ROC) curve was plotted for telomere length as a diagnostic marker for high-grade dysplasia.

Results

Telomere lengths were comparable among the low- and high-grade dysplasia cases and controls, with means of 0.96, 0.96, and 0.92, respectively. The area under the ROC curve was 0.55 for telomere length as a diagnostic marker for high-grade dysplasia. Further adjustment for subject characteristics, including sex, age, smoking, drinking, hypertension, and body mass index did not improve the use of telomere length as a marker for ESD.

Conclusions

Telomere length of esophageal balloon cytology cells was not associated with ESCC precursor lesions. Therefore, telomere length shows little promise as an early detection marker for ESCC in esophageal balloon samples.

【 授权许可】

   
2013 Lin et al.; licensee BioMed Central Ltd.

【 图 表 】

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