Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital

BMC Microbiology

Juan A Saez-Nieto²  María J Medina-Pascual²  Ana Vindel¹  Noelia Garrido²  Montserrat Ortega²  Teresa Cabezas³  Sylvia Valdezate²  Pilar Villalón² 

[1]Laboratorio de Infecciones Intrahospitalarias Servicio de Bacteriología, Centro Nacional de Microbiología Instituto de Salud Carlos III, Madrid, Majadahonda, Spain

[2]Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda-Pozuelo km2, Madrid, 28220, Majadahonda, Spain

[3]Laboratorio de Biotecnología Hospital de Poniente El Ejido, Almería, Spain

关键词: Clone;    MLVA;    Sequence type;    PFGE;    A. baumannii;   

Others  :  1137360
DOI  :  10.1186/s12866-015-0383-y

 received in 2014-09-30, accepted in 2015-02-11,  发布年份 2015

【 摘 要 】

Background

Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones isolated in a single hospital over a twelve-year period.

Results

PFGE analysis of 405 A. baumannii-calcoaceticus complex isolates detected 15 A. baumannii endemic/epidemic PFGE types (EE1 to EE15) that grouped into five clusters: EE1-EE8, EE9, EE10, EE11 and EE12-EE15. The MLST sequence type (ST) distributions were: international clone II (ST-2) 60%, international clone III (ST-3) 26.7%, ST-15 6.7%, and ST-80 6.7%. MLVA-8_Orsay returned 17 allelic profiles. The large (L) VNTR marker profiles were fully concordant with the detected STs, and concordant with 14 up to 15 PFGE types. Imipenem resistance was detected in five PFGE types; the prevalence of the bla_OXA-58-like and bla_OXA-40-like genes was 60% and 40% respectively.

Conclusions

PFGE proved to be a vital tool for analysis of the temporal and spatial distribution of the clones. MLST and the VNTR L-markers grouped the isolates into clonal clusters. The wide diversity of MLVA small (S)-markers, however, did not permit clustering. The present results demonstrate the persistence of several endemic PFGE types in the hospital, the involvement of some of them in outbreaks, and the inter hospital transmission of extensively drug-resistant ST-15 and ST-80.

【 授权许可】

   
2015 Villalón et al.; licensee BioMed Central.

【 图 表 】

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