Elevated levels of dehydroepiandrosterone as a potential mechanism of dendritic cell impairment during pregnancy

BMC Immunology

Nataliya M Pasman²  Alexandr A Ostanin¹  Nataliya A Khonina¹  Tamara V Tyrinova¹  Nataliya V Seledtsova¹  Marina A Tikhonova¹  Olga Yu Leplina¹  Elena R Chernykh¹ 

[1] Laboratory of Cellular Immunotherapy, Research Institute of Fundamental and Clinical Immunology, 14, Yadrintsevskaya St., Novosibirsk, 630099, Russia;Novosibirsk State University, 2, Pirogova St., Novosibirsk, 630090, Russia

关键词: Tolerogenic activity;    Pregnancy;    Dehydroepiandrosterone sulfate (DHEAS);    Dendritic cells;   

Others  :  1123258
DOI  :  10.1186/s12865-014-0065-9

【 摘 要 】

Background

This study aimed to test the hypothesis that immune dysfunction and the increased risk of spontaneous abortion in pregnant women with hyperandrogenia (HA) are caused by the reduced tolerogenic potential of dendritic cells (DCs) that results from elevated levels of dehydroepiandrosterone sulfate (DHEAS).

Methods

The phenotypic and functional properties of monocyte-derived DCs generated from blood monocytes from non-pregnant women, women with a normal pregnancy, or pregnant women with HA, as well as the in vitro effects of DHEAS on DCs in healthy pregnant women were investigated.

Results

In a normal pregnancy, DCs were shown to be immature and are characterized by a reduced number of CD83⁺ and CD25⁺ DCs, the ability to stimulate type 2 T cell responses and to induce T cell apoptosis. By contrast, DCs from pregnant women with HA had a mature phenotype, were able to stimulate both type 1 (IFN-γ) and type 2 (IL-4) T cell responses, and were characterized by lower B7-H1 expression and cytotoxic activity against CD8⁺ T cells. The addition of DHEAS to cultures of DCs from healthy pregnant women induced the maturation of DCs and increased their ability to activate type 1 T cell responses.

Conclusion

Our data demonstrated the reduction in the tolerogenic potential of DCs from pregnant women with HA, and revealed new mechanisms involved in the hormonal regulation of DCs mediated by DHEAS.

【 授权许可】

   
2015 Chernykh et al.; licensee BioMed Central.

【 图 表 】

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