Anti-proliferative of physcion 8-O-β-glucopyranoside isolated from Rumex japonicus Houtt. on A549 cell lines via inducing apoptosis and cell cycle arrest

BMC Complementary and Alternative Medicine

Yu-Peng Yang¹  Qi-Chao Xie¹ 

[1] Department of Oncology, the Second Affiliated Hospital, Third Military Medical University, 183 Xinqiao main street, Chongqing 400037, China

关键词: Lung cancer;    Apoptosis;    Anti-proliferative activity;    Rumex japonicus Houtt;    Physcion 8-O-β-glucopyranoside;   

Others  :  1086359
DOI  :  10.1186/1472-6882-14-377

 received in 2013-09-13, accepted in 2014-09-30,  发布年份 2014

【 摘 要 】

Background

Lung cancers are leading causes of cancer death, and Rumex japonicus has been traditionally used in folk medicine as anti-microorganic, anti-inflammatory and anti-tumor agents. This study was designed to investigate the anti-proliferative activity of physcion 8-O-β-glucopyranoside (PG) isolated from Rumex japonicus Houtt. on A549 cell lines.

Methods

In our present study, PG was isolated and identified from the ethanol extracts of R. japonicus. MTT method was used to evaluate the anti-proliferative activity of PG on A549 cell lines, and cell cycle distribution assay, apoptosis assay, and western blot analysis in vitro were used to explore the possible mechanisms.

Results

From the results of our present study, cell viability was obviously inhibited by PG, in a dose- and time-dependent manner. Our results also suggested that the anti-proliferative effect of PG was related to cell cycle arrest at the G2/M phase through repression of cdc2 and Cyclin B1 protein expression. In addition, the results of apoptosis assay and western blot analysis indicated that the anti-proliferative activity could be related to apoptosis via up-regulating the expressions of Bax, caspase-3 and caspase-7, and down-regulating the expressions of Bcl-2.

Conclusions

In conclusion, the PG has significant anti-proliferative activity on A549 cell lines, and the possible mechanism was related to cell cycle arrest at the G2/M phase, and apoptosis via the regulations of Bax, Bcl-2, and caspase-3 and caspase-7.

【 授权许可】

   
2014 Xie and Yang; licensee BioMed Central Ltd.

【 图 表 】

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