Niche differentiation in nitrogen metabolism among methanotrophs within an operational taxonomic unit

BMC Microbiology

Kim Heylen³  Paul De Vos²  Peter Vandamme³  Nico Boon¹  David van der Ha¹  Sven Hoefman³ 

[1] Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium;BCCM/LMG Bacteria Collection, Ghent, Belgium;Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium

关键词: Detoxification;    Nitrogen assimilation;    Strain dependency;    Methylomonas lenta;    Methylomonas koyamae;    Methylomonas methanica;   

Others  :  1141530
DOI  :  10.1186/1471-2180-14-83

 received in 2013-12-23, accepted in 2014-03-27,  发布年份 2014

【 摘 要 】

Background

The currently accepted thesis on nitrogenous fertilizer additions on methane oxidation activity assumes niche partitioning among methanotrophic species, with activity responses to changes in nitrogen content being dependent on the in situ methanotrophic community structure Unfortunately, widely applied tools for microbial community assessment only have a limited phylogenetic resolution mostly restricted to genus level diversity, and not to species level as often mistakenly assumed. As a consequence, intragenus or intraspecies metabolic versatility in nitrogen metabolism was never evaluated nor considered among methanotrophic bacteria as a source of differential responses of methane oxidation to nitrogen amendments.

Results

We demonstrated that fourteen genotypically different Methylomonas strains, thus distinct below the level at which most techniques assign operational taxonomic units (OTU), show a versatile physiology in their nitrogen metabolism. Differential responses, even among strains with identical 16S rRNA or pmoA gene sequences, were observed for production of nitrite and nitrous oxide from nitrate or ammonium, nitrogen fixation and tolerance to high levels of ammonium, nitrate, and hydroxylamine. Overall, reduction of nitrate to nitrite, nitrogen fixation, higher tolerance to ammonium than nitrate and tolerance and assimilation of nitrite were general features.

Conclusions

Differential responses among closely related methanotrophic strains to overcome inhibition and toxicity from high nitrogen loads and assimilation of various nitrogen sources yield competitive fitness advantages to individual methane-oxidizing bacteria. Our observations proved that community structure at the deepest phylogenetic resolution potentially influences in situ functioning.

【 授权许可】

   
2014 Hoefman et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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