A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae)

BMC Research Notes

AHM Khurshid Alam²  M Nurul Haque Mollah³  Shamima Nasrin²  Golam Sadik²  Mamunur Rashid²  Mosharrof Hossain⁵  Md Badrul Islam¹  Shahnaj Parvin²  Proma Khandokhar⁴  Shafiqul Islam²  Aziz Abdur Rahman²  Muhammad Ali Khan² 

[1] Senior Scientific Officer, BCSIR, Rajshahi, 6205, Bangladesh;Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh;Department of Statistics, University of Rajshahi, Rajshahi, 6205, Bangladesh;Department of Pharmaceutical and Biological Sciences, UCL School of Pharmacy, London, UK;Department of Zoology, University of Rajshahi, Rajshahi, 6205, Bangladesh

关键词: Correlation and regression;    Antioxidant;    Oxidative stress;    Moraceae;    Morus alba;   

Others  :  1144991
DOI  :  10.1186/1756-0500-6-24

 received in 2012-10-02, accepted in 2013-01-16,  发布年份 2013

【 摘 要 】

Background

Antioxidants play an important role to protect damage caused by oxidative stress (OS). Plants having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties and phenolic contents (total phenols, flavonoids, flavonols and proanthrocyanidins) of methanolic extracts from Morus alba (locally named as Tut and commonly known as white mulberry) stem barks (TSB), root bark (TRB), leaves (TL) and fruits (TF) to make a statistical correlation between phenolic contents and antioxidant potential.

Methods

The antioxidant activities and phenolic contents of methanolic extractives were evaluated by in vitro standard method using spectrophotometer. The antioxidant activities were determined by total antioxidant capacity, DPPH (1,1-diphenyl-2-picrylhydrazine) radical scavenging assay, hydroxyl radical scavenging assay, ferrous reducing antioxidant capacity and lipid peroxidation inhibition assay methods.

Results

Among the extracts, TSB showed the highest antioxidant activity followed by TRB, TF and TL. Based on DPPH and hydroxyl radical scavenging activity, the TSB extract was the most effective one with IC₅₀ 37.75 and 58.90 μg/mL, followed by TRB, TF and TL with IC₅₀ 40.20 and 102.03; 175.01 and 114.63 and 220.23 and 234.63 μg/mL, respectively. The TSB extract had the most potent inhibitory activity against lipid peroxidation with IC₅₀ 145.31 μg/mL. In addition, the reducing capacity on ferrous ion was in the following order: TSB > TRB > TL > TF. The content of phenolics, flavonoids, flavonols and proanthocyanidins of TSB was found to be higher than other extractives.

Conclusion

The results indicate high correlation and regression (p-value <0 .001) between phenolic contents and antioxidant potentials of the extracts, hence the Tut plant could serve as effective free radical inhibitor or scavenger which may be a good candidate for pharmaceutical plant-based products. However, further exploration is necessary for effective use in both modern and traditional system of medicines.

【 授权许可】

   
2013 Khan et al.; licensee BioMed Central Ltd.

【 图 表 】

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