Differential proteomics and physiology of Pseudomonas putida KT2440 under filament-inducing conditions

BMC Microbiology

Rob Van Houdt⁴  Pierre Cornelis²  Natalie Leys⁴  Abram Aertsen³  Ruddy Wattiez¹  Baptiste Leroy¹  Aurélie Crabbé⁵ 

[1]Department of Proteomics and Microbiology, Interdisciplinary Center of Mass Spectrometry (CISMa), University of Mons (UMONS), Mons, Belgium

[2]Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, Flanders Institute for Biotechnology (VIB), Vrije Universiteit Brussel, Brussels, Belgium

[3]Laboratory of Food Microbiology and Leuven Food Science and Nutrition Research Centre, Centre for Food and Microbial Technology, Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Leuven, Belgium

[4]Unit of Microbiology, Expert Group Molecular and Cellular Biology, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK CEN), Mol, Belgium

[5]Present address: The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, 1001 S. McAllister Avenue, Tempe, AZ, 85287, USA

关键词: Stress resistance;    Shaking speed;    RecA;    SOS response;    Elongation;    Filamentation;    Pseudomonas putida KT2440;   

Others  :  1144857
DOI  :  10.1186/1471-2180-12-282

 received in 2012-06-22, accepted in 2012-11-16,  发布年份 2012

【 摘 要 】

Background

Pseudomonas putida exerts a filamentous phenotype in response to environmental stress conditions that are encountered during its natural life cycle. This study assessed whether P. putida filamentation could confer survival advantages. Filamentation of P. putida was induced through culturing at low shaking speed and was compared to culturing in high shaking speed conditions, after which whole proteomic analysis and stress exposure assays were performed.

Results

P. putida grown in filament-inducing conditions showed increased resistance to heat and saline stressors compared to non-filamented cultures. Proteomic analysis showed a significant metabolic change and a pronounced induction of the heat shock protein IbpA and recombinase RecA in filament-inducing conditions. Our data further indicated that the associated heat shock resistance, but not filamentation, was dependent of RecA.

Conclusions

This study provides insights into the altered metabolism of P. putida in filament-inducing conditions, and indicates that the formation of filaments could potentially be utilized by P. putida as a survival strategy in its hostile, recurrently changing habitat.

【 授权许可】

   
2012 Crabbé et al.; licensee BioMed Central Ltd.

【 图 表 】

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