Ubiquinol reduces gamma glutamyltransferase as a marker of oxidative stress in humans

BMC Research Notes

Frank Döring²  Thomas Menke³  Wolfgang Lieb⁴  Ute Nöthlings¹  Gunnar Jacobs⁴  Petra Niklowitz³  Simone Onur² 

[1] Institute of Nutrition and Food Science, Rheinische Friedrich Wilhelms University Bonn, Meckenheimer Allee 168, 53115 Bonn, Germany;Institute of Human Nutrition and Food Science, Division of Molecular Prevention, Christian Albrechts University Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel, Germany;Children’s Hospital of Datteln, University of Witten/Herdecke, Dr.-Friedrich-Steiner Str. 5, 45711 Datteln, Germany;Institute of Epidemiology and Biobank Popgen, Christian Albrechts University Kiel, Campus University Hospital Schleswig-Holstein, Niemannsweg 11, Haus 1, 24105 Kiel, Germany

关键词: Antioxidants;    Oxidative stress;    Liver enzymes;    Supplementation study;    Gene expression;    Ubiquinol;    Coenzyme Q₁₀;   

Others  :  1132303
DOI  :  10.1186/1756-0500-7-427

 received in 2014-02-27, accepted in 2014-06-23,  发布年份 2014

【 摘 要 】

Background

The reduced form of Coenzyme Q₁₀ (CoQ₁₀), ubiquinol (Q₁₀H₂), serves as a potent antioxidant in mitochondria and lipid membranes. There is evidence that Q₁₀H₂ protects against oxidative events in lipids, proteins and DNA. Serum gamma-glutamyltransferase (GGT) activity is associated with cardiovascular diseases. In a physiological range, activity of GGT is a potential early and sensitive marker of inflammation and oxidative stress.

In this study, we first examined the relationship between CoQ₁₀ status and serum GGT activity in 416 healthy participants between 19 and 62 years of age in a cross-sectional study (cohort I). In the second step, 53 healthy males (21–48 years of age; cohort II) underwent a 14-day Q₁₀H₂ supplementation (150 mg/d) to evaluate the effect of Q₁₀H₂ supplementation on serum GGT activity and GGT1 gene expression.

Findings

There was a strong positive association between CoQ₁₀ status and serum GGT activity in cohort I. However, a gender-specific examination revealed differences between male and female volunteers regarding the association between CoQ₁₀ status and serum GGT activity. Q₁₀H₂ supplementation (cohort II) caused a significant decrease in serum GGT activity from T₀ to T₁₄ (p < 0.001). GGT1 mRNA levels declined 1.49-fold after Q₁₀H₂ supplementation. Of note, other liver enzymes (i.e., aspartate aminotransferase, AST) were not affected by Q₁₀H₂ supplementation.

Conclusions

CoQ₁₀ level is positively associated with serum GGT activity. Supplementation with Q₁₀H₂ reduces serum GGT activity. This effect might be caused by gene expression. Overall, we provide preliminary evidence that higher Q₁₀H₂ levels improve oxidative stress via reduction of serum GGT activity in humans.

Trial registration

Current Controlled TrialsISRCTN26780329.

【 授权许可】

   
2014 Onur et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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