Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells

BMC Molecular Biology

Bin Li¹  Xiaohe Lu²  Ning Kong¹ 

[1] Department of Ophthalmology, Guangzhou Panyu Central Hospital, Guangzhou 510280, Guangdong Province, China;Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China

关键词: IGF-1;    Apoptosis;    Oxidative stress;    miR-100;    Retinal ganglion;   

Others  :  1090203
DOI  :  10.1186/s12867-014-0025-1

 received in 2014-08-03, accepted in 2014-10-22,  发布年份 2014

【 摘 要 】

Background

Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells.

Results

Rat RGC-5 cells were cultured in plates and H₂O₂ was added to induce oxidative stress. TUNEL assay and qRT-PCR showed H₂O₂ induced apoptosis and up-regulated miR-100 in a dose-dependent manner in RGC-5 cells. Conversely, lentiviral-mediated miR-100 down-regulation protected H₂O₂ induced apoptosis, promoted neurite growth and activated AKT/ERK and TrkB pathways through phosphorylation. Luciferase assay confirmed that IGF1R was directly regulated by miR-100 in RGC-5 cells, and siRNA-mediated IGF1R knockdown activated AKT protein through phosphorylation, down-regulated miR-100, therefore exerted a protective effect on RGC-5 apoptosis.

Conclusion

Down-regulating miR-100 is an effective method to protect H₂O₂ induced apoptosis in RGC-5 cells, possible associated with IGF1R regulation.

【 授权许可】

   
2014 Kong et al.; licensee BioMed Central Ltd.

【 图 表 】

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