Human Neuroepithelial Cells Express NMDA Receptors

BMC Neuroscience

JS Alexander⁴  A Minagar¹  A Long⁴  B Cappell⁴  I Chandler⁴  A Nanda³  A Warren⁴  J Houghton⁴  TH Jackson⁴  M Fowler²  Christopher D Sharp⁴ 

[1] Department of Neurology, LSU Health Sciences Center, Shreveport, LA, USA;Department of Pathology, LSU Health Sciences Center, Shreveport, LA, USA;Department of Neurosurgery, LSU Health Sciences Center, Shreveport, LA, USA;Department of Molecular and Cellular Physiology, LSU Health Sciences Center, Shreveport, LA, USA

关键词: Brain;    Human;    Neuroepithelial;    NMDA;    Glutamate;   

Others  :  1223206
DOI  :  10.1186/1471-2202-4-28

 received in 2003-03-14, accepted in 2003-11-13,  发布年份 2003

【 摘 要 】

L-glutamate, an excitatory neurotransmitter, binds to both ionotropic and metabotropic glutamate receptors. In certain parts of the brain the BBB contains two normally impermeable barriers: 1) cerebral endothelial barrier and 2) cerebral epithelial barrier. Human cerebral endothelial cells express NMDA receptors; however, to date, human cerebral epithelial cells (neuroepithelial cells) have not been shown to express NMDA receptor message or protein. In this study, human hypothalamic sections were examined for NMDA receptors (NMDAR) expression via immunohistochemistry and murine neuroepithelial cell line (V1) were examined for NMDAR via RT-PCR and Western analysis. We found that human cerebral epithelium express protein and cultured mouse neuroepithelial cells express both mRNA and protein for the NMDA receptor. These findings may have important consequences for neuroepithelial responses during excitotoxicity and in disease.

【 授权许可】

   
2003 Sharp et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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【 参考文献 】

• [1]Nicoll RA, Oliet SHR, Malenka RC: NMDA Receptor-Dependent and Metabotropic Glutamate Receptor-Dependent Forms of Long-Term Depression Coexist in CA1 Hippocampal Pyramidal Cells. Neurobiol Learn Mem 1998, 70:62-72.
• [2]Hollmann M, O'Shea-Greenfield A, Rogers SW, Heinemann S: Cloning by functional expression of a member of the glutamate receptor family. Nature 1989, 342:643-648.
• [3]Bos KD, Slump P: Determination of glutamine and glutamate in plasma of men and women by ion exchange chromatography. Clin Chim Acta 1985, 152:205-211.
• [4]Fonnum F: Glutamate: a neurotransmitter in mammalian brain. J Neurochem 1984, 42:1-11.
• [5]Coyle JT, Bird SJ, Evans RH, Gulley RL, Nadler JV, Nicklas WJ, et al.: Excitatory amino acid neurotoxins: selectivity, specificity, and mechanisms of action. Based on an NRP one-day conference held June 30, 1980. Neurosci Res Program Bull 1981, 19:1-427.
• [6]Coyle JT, Puttfarcken P: Oxidative stress, glutamate, and neurodegenerative disorders. Science 1993, 262:689-695.
• [7]Bogaert L, Scheller D, Moonen J, Sarre S, Smolders I, Ebinger G, et al.: Neurochemical changes and laser Doppler flowmetry in the endothelin-1 rat model for focal cerebral ischemia. Brain Res 2000, 887:266-275.
• [8]Lipton SA, Rosenberg PA: Excitatory amino acids as a final common pathway for neurologic disorders. N Engl J Med 1994, 330:613-622.
• [9]Olney JW: Glutamate, a neurotoxic transmitter. J Child Neurol 1989, 4:218-226.
• [10]Kempski O, von Andrian U, Schurer L, Baethmann A: Intravenous glutamate enhances edema formation after a freezing lesion. Adv Neurol 1990, 52:219-223.
• [11]Kempski O: Cerebral edema. Semin Nephrol 2001, 21:303-307.
• [12]Rosenberg GA: Ischemic brain edema. Prog Cardiovasc Dis 1999, 42:209-216.
• [13]Abbott NJ: Inflammatory mediators and modulation of blood-brain barrier permeability. Cell Mol Neurobiol 2000, 20:131-147.
• [14]Gorgulu A, Kins T, Cobanoglu S, Unal F, Izgi NI, Yanik B, et al.: Reduction of edema and infarction by Memantine and MK-801 after focal cerebral ischaemia and reperfusion in rat. Acta Neurochir (Wien) 2000, 142:1287-1292.
• [15]Reynolds IJ, Hastings TG: Glutamate induces the production of reactive oxygen species in cultured forebrain neurons following NMDA receptor activation. J Neurosci 1995, 15:3318-3327.
• [16]Brahma B, Forman RE, Stewart EE, Nicholson C, Rice ME: Ascorbate inhibits edema in brain slices. J Neurochem 2000, 74:1263-1270.
• [17]Buckman JF, Reynolds IJ: Spontaneous changes in mitochondrial membrane potential in cultured neurons. J Neurosci 2001, 21:5054-5065.
• [18]Urushitani M, Nakamizo T, Inoue R, Sawada H, Kihara T, Honda K, et al.: N-methyl-D-aspartate receptor-mediated mitochondrial Ca(2+) overload in acute excitotoxic motor neuron death: a mechanism distinct from chronic neurotoxicity after Ca(2+) influx. J Neurosci Res 2001, 63:377-387.
• [19]Marone M, Quinones-Jenab V, Meiners S, Nowakowski RS, Ho SY, Geller HM: An immortalized mouse neuroepithelial cell line with neuronal and glial phenotypes. Dev Neurosci 1995, 17:311-323.
• [20]Alexander JS, Blaschuk OW, Haselton FR: An N-cadherin-like protein contributes to solute barrier maintenance in cultured endothelium. J Cell Physiol 156(3):610-618.
• [21]Ban Y, Wilt SD, Rizzolo LJ: Two secreted retinal factors regulate different stages of development of the outer blood-retinal barrier. Brain Res Dev Brain Res 119(2):259-267.
• [22]Wegener J, Sieber M, Galla HJ: Impedance analysis of epithelial and endothelial cell monolayers cultured on gold surfaces. J Biochem Biophys Methods 1996, 32(3):151-70.
• [23]Nassif X, Bourdoulous S, Eugene E, Couraud PO: How do extracellular pathogens cross the blood-brain barrier? Trends Microbiol 2002, 10:227-232.
• [24]Sharp CD, Hines IN, Warren AC, Elrod JW, Jennings M, Laroux FS, et al.: NMDA receptor (NMDAR1) dependent oxygen radicals disrupt junctional barrier in human brain endothelial monolayers. FASEB J 2002, 16(5):A1124.
• [25]Rotrosen D, Gallin JI: Histamine type I receptor occupancy increases endothelial cytosolic calcium, reduces F-actin, and promotes albumin diffusion across cultured endothelial monolayers. J Cell Biol 1986, 103:2379-2387.
• [26]Kim CS, Virella A, Braunberg RC, Ross IA, Matthews RN, Johnson W, et al.: Kinetic analysis of glutamate transport by the miniswine choroid plexus in vitro. Brain Res 1996, 709:59-64.
• [27]al Sarraf H, Preston JE, Segal MB: Acidic amino acid accumulation by rat choroid plexus during development. Brain Res Dev Brain Res 1997, 102:47-52.