Expression of extra-cellular levansucrase in Pseudomonas syringae is controlled by the in planta fitness-promoting metabolic repressor HexR

BMC Microbiology

Matthias S Ullrich¹  Daniel Pletzer¹  Gabriela Alfaro-Espinoza¹  Nehaya Al-Karablieh²  Abhishek Srivastava¹  Daria Zhurina¹  Shaunak Khandekar¹  Khaled Abdallah¹  Amna Mehmood¹ 

[1] Molecular Life Science Research Center, Jacobs University Bremen, Campus Ring 1, Bremen 28759, Germany;Hamdi Mango Center for Scientific Research, The University of Jordan, Amman 11942, Jordan

关键词: HexR;    Hexose metabolism;    Levansucrase;    Pseudomonas syringae;    Soybean;    Bacterial blight;    Plant pathogen;   

Others  :  1137359
DOI  :  10.1186/s12866-015-0349-0

 received in 2014-10-30, accepted in 2015-01-15,  发布年份 2015

【 摘 要 】

Background

Pseudomonas syringae pv. glycinea PG4180 causes bacterial blight on soybean plants and enters the leaf tissue through stomata or open wounds, where it encounters a sucrose-rich milieu. Sucrose is utilized by invading bacteria via the secreted enzyme, levansucrase (Lsc), liberating glucose and forming the polyfructan levan. P. syringae PG4180 possesses two functional lsc alleles transcribed at virulence-promoting low temperatures.

Results

We hypothesized that transcription of lsc is controlled by the hexose metabolism repressor, HexR, since potential HexR binding sites were identified upstream of both lsc genes. A hexR mutant of PG4180 was significantly growth-impaired when incubated with sucrose or glucose as sole carbon source, but exhibited wild type growth when arabinose was provided. Analyses of lsc expression resulted in higher transcript and protein levels in the hexR mutant as compared to the wild type. The hexR mutant’s ability to multiply in planta was reduced. HexR did not seem to impact hrp gene expression as evidenced by the hexR mutant’s unaltered hypersensitive response in tobacco and its unmodified protein secretion pattern as compared to the wild type under hrp-inducing conditions.

Conclusions

Our data suggested a co-regulation of genes involved in extra-cellular sugar acquisition with those involved in intra-cellular energy-providing metabolic pathways in P. syringae.

【 授权许可】

   
2015 Mehmood et al.; licensee BioMed Central.

【 图 表 】

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