Isolation of methyl gamma linolenate from spirulina platensis using flash chromatography and its apoptosis inducing effect

BMC Complementary and Alternative Medicine

Chaitanya M. V. N. L.²  Dhanabal S. P.³  Jubie S.¹ 

[1] Department of Pharmaceutical chemistry, JSS College of Pharmacy, JSS University, Mysore, India;Research Associate, Department of Pharmacognosy and Phytopharmacy, JSS College of Pharmacy, Ooty-643001 JSS University, Mysore, India;Department of Pharmacognosy and Phytopharmacy, J. S. S. College of Pharmacy, Rock lands, Tamilnadu, Ooty-643 001, India

关键词: Flash chromatography;    Cytotoxicity;    Gamma linolenic acid (GLA);    Lung cancer;    Spirulina platensis;   

Others  :  1222060
DOI  :  10.1186/s12906-015-0771-8

 received in 2014-11-12, accepted in 2015-07-08,  发布年份 2015

【 摘 要 】

Background

Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect against human lung carcinoma A- 549 cell lines.

Methods

Gamma linolenic acid is an important omega-6 polyunsaturated fatty acid (PUFA) of medicinal interest was isolated from microalgae Spirulina platensis using flash chromatography system (Isolera system) as its methyl ester. The isolated methyl gamma linolenate was characterized by IR,¹ H NMR,¹³ C NMR and mass spectral analysis and the data were consistent with the structure.

Results

The percentage yield of isolated methyl gamma linolenate is found to be 71 % w/w, which is a very good yield in comparison to other conventional methods. It was subjected to in-vitro cytotoxic screening on A-549 lung cancer cell lines using SRB assay and result was compared with standard rutin.

Conclusion

It may be concluded that the Flash chromatography system plays a major role in improving the yield for theisolation of methyl gamma linoleate from Spirulina platensis and the isolated molecule is a potent cytotoxicagent towards human lung carcinoma cell lines, however it may be further taken up for an extensive study.

【 授权许可】

   
2015 S. et al.

【 图 表 】

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