Proteins and peptides in parotid saliva of irradiated patients compared to that of healthy controls using SELDI-TOF-MS

BMC Research Notes

Cor van Loveren²  Erika Silletti¹  Arnoud Loof³  Raymond G. Schipper⁵  Johannes J. de Soet²  Bernd W. Brandt²  Coen N. Rasch⁴  Alexa M. G. A. Laheij² 

[1] NIZO Food Research B.V., Ede, 6710 BA, The Netherlands;Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, Amsterdam, 1081 LA, The Netherlands;Central Laboratory for Haematology, Radboud University Nijmegen Medical Centre Post 476, Nijmegen, 6500 HB, The Netherlands;Department of Radiation Oncology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, Amsterdam, 1066 CX, The Netherlands;Top Institute Food and Nutrition, Wageningen, 6700 AN, The Netherlands

关键词: SELDI-TOF-MS;    Parotid gland;    Proteomics;    Saliva;    Head and neck cancer;   

Others  :  1232829
DOI  :  10.1186/s13104-015-1641-7

 received in 2014-07-28, accepted in 2015-10-27,  发布年份 2015

【 摘 要 】

Background

Radiotherapy to the head and neck area damages the salivary glands. As a consequence hyposalivation may occur, but also the protein composition of saliva may be affected possibly compromising oral health. The aim of our study was to compare the relative abundance of proteins and peptides in parotid saliva of irradiated patients to that of healthy controls.

Methods

Using Lashley cups and citric acid, saliva from the parotid glands was collected from nine irradiated patients and ten healthy controls. The samples were analyzed with SELDI-TOF-MS using a NP20 and IMAC-30 chip in the molecular weight range of 1–30 kDa.

Results

On the NP20 chip 61 (out of 217) and on the IMAC-30 chip 32 (out of 218) peaks differed significantly in intensity between the saliva of the irradiated patients and healthy controls. 55 % of the significant peaks showed higher intensity and 45 % showed lower intensity in the saliva of irradiated patients. The peaks may represent, amongst others, the salivary proteins lysozyme, histatins, cystatin, protein S100 and PRP’s.

Conclusions

Large differences were found in the relative abundance of a wide range of proteins and peptides in the parotid saliva of irradiated patients compared to healthy controls.

【 授权许可】

   
2015 Laheij et al.

【 图 表 】

Fig.1.

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