Aging is associated with increased activities of matrix metalloproteinase-2 and -9 in tenocytes

BMC Musculoskeletal Disorders

Wen-Chung Tsai¹  Chien-Hung Chen³  Max J-L Chen³  Katie Pei-Hsuan Wu³  Jong-Hwei S Pang²  Tung-Yang Yu² 

[1] College of Medicine, Change Gung University, Kwei-Shan Tao-Yuan, Taiwan;Graduate Institute of Clinical Medical Sciences, Chang Gung University, Kwei-Shan Tao-Yuan, Taiwan;Departement of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Linkou, Taiwan

关键词: Transforming growth factor-beta 1;    Tenocytes;    Matrix metalloproteinase;    Collagen;    Aging;   

Others  :  1134287
DOI  :  10.1186/1471-2474-14-2

 received in 2012-05-21, accepted in 2012-12-23,  发布年份 2013

【 摘 要 】

Background

Most tendon pathology is associated with degeneration, which is thought to involve cyclic loading and cumulative age-related changes in tissue architecture. However, the association between aging and degeneration of the extracellular matrix (ECM) in tendons has not been investigated extensively.

Methods

We examined tenocytes from Achilles tendons taken from rats of three different ages (2, 12, and 24 months). Tenocyte viability was assessed using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Quantitative real-time polymerase chain reaction (PCR) was used to determine the levels of mRNAs that encode type-I collagen, matrix metalloproteinase (MMP)-2 and −9, tissue inhibitor of metalloproteinase (TIMP)-1 and −2 and transforming growth factor (TGF)-β1. Gelatin zymography was used to evaluate the enzymatic activities of MMP-2 and −9. Furthermore, the concentration of TGF-β1 in conditioned medium was evaluated using enzyme-linked immunosorbent assay (ELISA).

Results

The results of the MTT assay showed that the number of viable tenocytes decreased with age. No differences were observed in the levels of mRNAs that encode type-I collagen and TGF-β1 among the three age groups, and the TGF-β1 concentration did not change with age. However, mRNAs that encode MMP-2 and −9 were significantly more abundant in tenocytes from the aging group, and gelatin zymography revealed that the enzymatic activities of MMP-2 and −9 also increased significantly with age. Furthermore, as compared with young group, mRNAs that encode TIMP-1 and −2 were significantly decreased in tenocytes from the aging group.

Conclusions

Activities of MMP-2 and MMP-9 in tenocytes increase with age. This might provide a mechanistic explanation of how aging contributes to tendinopathy or tendon rupture with age.

【 授权许可】

   
2013 Yu et al.; licensee BioMed Central Ltd.

【 图 表 】

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