Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae)

BMC Complementary and Alternative Medicine

Ajit Kumar Saxena³  Payare Lal Sangwan²  Nidhi Gupta²  Sadhna Sharma³  Gurpreet Singh¹  Meenu Katoch¹ 

[1] Microbial Biotechnology Department, Indian Institute of Integrative Medicine, Jammu, India;Natural Product Chemistry, Indian Institute of Integrative Medicine, Jammu 180001, India;Cancer Pharmacology Department, Indian Institute of Integrative Medicine, Jammu, India

关键词: HCT-116;    Cytotoxicity;    Antimicrobial activity;    MTT assay;    Endophytic fungi;    Bacopa monnieri;   

Others  :  1220269
DOI  :  10.1186/1472-6882-14-52

 received in 2013-09-21, accepted in 2014-02-06,  发布年份 2014

【 摘 要 】

Background

Endophytes, which reside in plant tissues, have the potential to produce novel metabolites with immense benefits for health industry. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell were investigated.

Methods

Endophytic fungi were isolated from the Bacopa monnieri. Extracts from liquid cultures were tested for cytotoxicity against a number of cancer cell lines using the MTT assay. Antimicrobial activity was determined using the micro dilution method.

Results

22% of the examined extracts showed potent (IC₅₀ of <20 μg/ml) cytotoxic activity against HCT-116 cell line. 5.5%, 11%, 11% of the extracts were found to be cytotoxic for MCF-7, PC-3, and A-549 cell lines respectively. 33% extracts displayed antimicrobial activity against at least one test organism with MIC value 10–100 μg/ml. The isolate B9_Pink showed the most potent cytotoxic activity for all the cell lines examined and maximum antimicrobial activity against the four pathogens examined which was followed by B19.

Conclusions

Results indicated the potential for production of bioactive agents from endophytes of Bacopa monnieri.

【 授权许可】

   
2014 Katoch et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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