Evaluation and differentiation of the Betulaceae birch bark species and their bioactive triterpene content using analytical FT-vibrational spectroscopy and GC-MS

Chemistry Central Journal

Simona Cîntă-Pînzaru²  Cristina A Dehelean³  Codruta Soica³  Monica Culea¹  Florin Borcan³ 

[1] Biomedical Physics Department, Babeş-Bolyai University, 1st M. Kogălniceanu Str., Cluj-Napoca, 400084, Romania

[2] Molecular Spectroscopy Dept., Babeş-Bolyai University, 1st M. Kogălniceanu Str., Cluj-Napoca, 400084, Romania

[3] Faculty of Pharmacy, Victor Babeş University of Medicine and Pharmacy, 2nd Eftimie Murgu Sq., Timişoara, 300041, Romania

关键词: FT-IR;    FT-Raman;    Lupeol;    Betulinic acid;    Betulin;    Natural extracts;   

Others  :  788109
DOI  :  10.1186/1752-153X-6-67

 received in 2012-04-26, accepted in 2012-07-18,  发布年份 2012

【 摘 要 】

Background

Aiming to obtain the highest triterpene content in the extraction products, nine bark samples from the forest abundant flora of Apuseni Mountains, Romania were Raman spectroscopically evaluated. Three different natural extracts from Betula pendula Roth birch bark have been obtained and characterized using Fourier transform vibrational spectra.

Results

This study shows that principal components of the birch tree extract can be rapidly recognized and differentiated based on their vibrational fingerprint band shape and intensity. The vibrational spectroscopy results are supported by the GC-MS data. Based on IR and Raman analysis, one can conclude that all the extracts, independent on the solvent(s) used, revealed dominant betulin species, followed by lupeol.

Conclusions

Since Raman measurements could also be performed on fresh plant material, we demonstrated the possibility to apply the present results for the prediction of the highest triterpene content in bark species, for the selection of harvesting time or individual genotypes directly in the field, with appropriate portable Raman equipment.

【 授权许可】

   
2012 Cinta-Pinzaru et al.; licensee Chemistry Central Ltd.

【 图 表 】

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