Chemical quantification and antioxidant assay of four active components in Ficus hirta root using UPLC-PAD-MS fingerprinting combined with cluster analysis

Chemistry Central Journal

Tao Yi²  Qilei Chen²  Xicheng He²  Suiwai So²  Yuenling Lo²  Lanlan Fan¹  Jun Xu²  Yina Tang²  Jianye Zhang²  Zhongzhen Zhao²  Hubiao Chen² 

[1] Guangxi Botanical Garden of Medicinal Plant, Nanning, China

[2] School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, Hong Kong, P. R. China

关键词: Antioxidant capacity;    Fingerprinting;    Chemical quantification;    UPLC-PAD-MS;    Ficus hirta;   

Others  :  787878
DOI  :  10.1186/1752-153X-7-115

 received in 2013-05-30, accepted in 2013-07-05,  发布年份 2013

【 摘 要 】

Background

Root of Ficus hirta (RFH) is widely consumed in China as a plant-derived popular food. However, contents of the active constituents of RFH are unknown, and the chemical as well as bioactive properties of RFH may be affected by growing area. In order to ensure the standard efficacy of health products made with RFH, its active constituents should firstly be determined and, secondly, a means of assessing samples for their contents of these constituents is needed.

Results

Four active components, including two coumarins, namely psoralen and bergapten, and two flavonoids, namely luteolin and apigenin, in twenty RFH samples were quantified using a new ultra performance liquid chromatography coupled with photodiode array detector and mass spectrometry (UPLC-PAD-MS) method, and the content level in descending order was psoralen > bergapten > luteolin > apigenin. Chromatographic fingerprint similarity evaluation and cluster analysis were used to assess geographical origin of RFH, and the results revealed a high level of similarity for the tested RFH samples obtained from Hainan, Guangdong, Guangxi provinces and Hong Kong. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was conducted to evaluate the antioxidant potencies of the four components, and the results clearly demonstrated that luteolin was most effective; apigenin exhibited a moderate potency, whereas psoralen and bergapten possessed little effect against free radical reactions. Structure-activity relationship of the components was elucidated, and the 3′-hydroxyl group of luteolin was found to be directly responsible for its antioxidant activity.

Conclusion

The present UPLC-PAD-MS method and DPPH radical scavenging assay performed well for the purpose of constituent quantification and antioxidant assay. Global profiles were highly similar for RFH samples from different origins. Both the coumarins and flavonoids were involved in the health benefit of RFH.

【 授权许可】

   
2013 Yi et al.; licensee Chemistry Central Ltd.

【 图 表 】

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