Characterization and determination of six flavonoids in the ethnomedicine “Dragon’s Blood” by UPLC-PAD-MS

Chemistry Central Journal

Tao Yi¹  Yina Tang¹  Jianye Zhang¹  Zhongzhen Zhao¹  Zhijun Yang¹  Hubiao Chen¹ 

[1] School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, P. R. China

关键词: Quality evaluation;    Characterization;    UPLC-PAD-MS;    Daemonorops draco;    Dragon's Blood;   

Others  :  788060
DOI  :  10.1186/1752-153X-6-116

 received in 2012-09-10, accepted in 2012-10-08,  发布年份 2012

【 摘 要 】

Background

“Dragon’s Blood” (DB) has long been used as an ethnomedicine in China to invigorate blood circulation for the treatment of traumatic injuries, blood stasis and pain. To comprehensively assess the quality of DB medicine, a precise and accurate method that can rapidly separate, characterize and quantify multiple active components of DB is crucial.

Results

An ultra performance liquid chromatography (UPLC) coupled with photodiode array detection (PAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for characterization and determination of six flavonoids in DB. A comprehensive validation of the developed method was conducted, and confirmed that the method presented good sensitivity, precision and accuracy. All linear regressions were acquired with R² > 0.99, and the limits of detection ranged from 0.06 to 0.83 ng. The relative standard deviation (RSD) values were found to be within the range 1.4–3.8% for the method repeatability test. Recovery studies for the quantified compounds were found to be within the range 94.2–102.8% with RSD less than 4.9%. DB samples collected from different geographical regions were analyzed by the present method, and the results demonstrated that the contents of the six flavonoids in DB samples varied significantly. Three major active components among the six flavonoids, namely dracorhodin, (2S)-5-methoxyflavan-7-ol and (2S)-5-methoxy-6-methylflavan-7-ol, are suggested as the index for DB quality evaluation.

Conclusions

Overall, the present hyphenation method is highly efficient and reliable, and hence suitable for the characterization and determination of the flavonoids of DB ethnomedicine.

【 授权许可】

   
2012 Yi et al.; licensee Chemistry Central Ltd.

【 图 表 】

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