Comparison of sugar content for ionic liquid pretreated Douglas-fir woodchips and forestry residues

Biotechnology for Biofuels

Seema Singh³  Blake A Simmons³  Vitalie Stavila³  Samuel P Plummer¹  Aaron M Socha² 

[1]Deconstruction Division, Joint BioEnergy Institute, 5885 Hollis Avenue, Emeryville, CA 94608, USA

[2]Department of Chemistry and Chemical Technology, Bronx Community College, Bronx, NY 10453, USA

[3]Biological and Materials Science Center, Sandia National Laboratories, 7011 East Avenue, Livermore, CA 94551, USA

关键词: 1-ethyl-3-methylimidazolium acetate;    Forestry residues;    Woodchips;    Softwood;    Douglas-fir;    Ionic liquid pretreatment;    Biomass pretreatment;    Lignocellulose;   

Others  :  798078
DOI  :  10.1186/1754-6834-6-61

 received in 2013-01-08, accepted in 2013-04-29,  发布年份 2013

【 摘 要 】

Background

The development of affordable woody biomass feedstocks represents a significant opportunity in the development of cellulosic biofuels. Primary woodchips produced by forest mills are considered an ideal feedstock, but the prices they command on the market are currently too expensive for biorefineries. In comparison, forestry residues represent a potential low-cost input but are considered a more challenging feedstock for sugar production due to complexities in composition and potential contamination arising from soil that may be present. We compare the sugar yields, changes in composition in Douglas-fir woodchips and forestry residues after pretreatment using ionic liquids and enzymatic saccharification in order to determine if this approach can efficiently liberate fermentable sugars.

Results

These samples were either mechanically milled through a 2 mm mesh or pretreated as received with the ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate [C₂mim][OAc] at 120°C and 160°C. IL pretreatment of Douglas-fir woodchips and forestry residues resulted in approximately 71-92% glucose yields after enzymatic saccharification. X-ray diffraction (XRD) showed that the pretreated cellulose was less crystalline after IL pretreatment as compared to untreated control samples. Two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR) revealed changes in lignin and hemicellulose structure and composition as a function of pretreatment. Mass balances of sugar and lignin streams for both the Douglas-fir woodchips and forestry residues throughout the pretreatment and enzymatic saccharification processes are presented.

Conclusions

While the highest sugar yields were observed with the Douglas-fir woodchips, reasonably high sugar yields were obtained from forestry residues after ionic liquid pretreatment. Structural changes to lignin, cellulose and hemicellulose in the woodchips and forestry residues of Douglas-fir after [C₂mim][OAc] pretreatment are analyzed by XRD and 2D-NMR, and indicate that significant changes occurred. Irrespective of the particle sizes used in this study, ionic liquid pretreatment successfully allowed high glucose yields after enzymatic saccharification. These results indicate that forestry residues may be a more viable feedstock than previously thought for the production of biofuels.

【 授权许可】

   
2013 Socha et al.; licensee BioMed Central Ltd.

【 图 表 】

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