Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

Biotechnology for Biofuels

Ulrich Commandeur²  Rainer Fischer³  Holger Klose⁴  Dirk Heesel²  Johannes Klinger²  Megan Garvey¹  Camilla Lambertz² 

[1]Present address: School of Medicine, Deakin University, CSIRO Australian Animal Health Laboratory, 5 Portarlington Rd, Newcomb 3219, VIC, Australia

[2]Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, Aachen, 52074, Germany

[3]Fraunhofer Institute for Molecular Biology and Applied Ecology, Forckenbeckstrasse 6, Aachen, 52074, Germany

[4]Present address: Institute for Botany and Molecular Genetics, RWTH Aachen University, Worringerweg 3, Aachen, 52074, Germany

关键词: Industrial processing;    Consolidated bioprocessing;    Cellulosome;    Heterologous expression;    Cellulase;   

Others  :  1084341
DOI  :  10.1186/s13068-014-0135-5

 received in 2014-06-05, accepted in 2014-09-03,  发布年份 2014

【 摘 要 】

Second generation biofuel development is increasingly reliant on the recombinant expression of cellulases. Designing or identifying successful expression systems is thus of preeminent importance to industrial progress in the field. Recombinant production of cellulases has been performed using a wide range of expression systems in bacteria, yeasts and plants. In a number of these systems, particularly when using bacteria and plants, significant challenges have been experienced in expressing full-length proteins or proteins at high yield. Further difficulties have been encountered in designing recombinant systems for surface-display of cellulases and for use in consolidated bioprocessing in bacteria and yeast. For establishing cellulase expression in plants, various strategies are utilized to overcome problems, such as the auto-hydrolysis of developing plant cell walls. In this review, we investigate the major challenges, as well as the major advances made to date in the recombinant expression of cellulases across the commonly used bacterial, plant and yeast systems. We review some of the critical aspects to be considered for industrial-scale cellulase production.

【 授权许可】

   
2014 Lambertz et al.; licensee BioMed Central Ltd.

【 图 表 】

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