Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity

Biotechnology for Biofuels

Masaki Muto¹  Masayoshi Tanaka³  Yue Liang³  Tomoko Yoshino³  Mitsufumi Matsumoto²  Tsuyoshi Tanaka¹ 

[1] JST, CREST, Sanbancho 5, Chiyoda-ku 102-0075, Tokyo, Japan

[2] Biotechnology Laboratory, Electric Power Development Co., Ltd., Yanagisaki-machi, Wakamatsu-ku 808-0111, Kitakyusyu, Japan

[3] Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei 184-8588, Tokyo, Japan

关键词: Metabolic engineering;    Biodiesel fuel;    Glycerol kinase;    Mixotrophic cultivation;    Fistulifera solaris;    Oleaginous microalgae;   

Others  :  1089695
DOI  :  10.1186/s13068-014-0184-9

 received in 2014-05-09, accepted in 2014-12-11,  发布年份 2015

【 摘 要 】

Background

Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity and biomass productivity by overcoming the growth retardation caused by decreased light penetration in high-density culture.

Results

Overexpression of the endogenous glycerol kinase (GK) gene in an oleaginous marine diatom, Fistulifera solaris JPCC DA0580, accelerates glycerol metabolism and improves lipid and biomass productivities. Two candidates were selected from a collection of 90 G418-resistant clones, based on growth and confirmation of genome integration. GK gene expression was higher in the selected clones (GK1_7 and GK2_16) than in the wild-type culture. The GK2_16 clone achieved a 12% increase in lipid productivity.

Conclusion

We have demonstrated the potential of metabolic engineering in oleaginous microalgae to improve lipid productivity. Metabolic engineering techniques can be used to optimize BDF production.

【 授权许可】

   
2015 Muto et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

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