Simultaneous determination of seven hydrophilic bioactive compounds in water extract of Polygonum multiflorum using pressurized liquid extraction and short-end injection micellar electrokinetic chromatography

Chemistry Central Journal

Ka-meng Lao³  Dong-qi Han²  Xiao-jia Chen³  Jing Zhao³  Tie-jie Wang²  Shao-ping Li¹ 

[1] School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, China

[2] Shenzhen Institute for Drug Control, 518057, Shenzhen, P. R. China

[3] State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China

关键词: Short-end injection;    Pressurized liquid extraction;    Polygonum multiflorum;    MEKC;   

Others  :  787947
DOI  :  10.1186/1752-153X-7-45

 received in 2013-01-25, accepted in 2013-02-28,  发布年份 2013

【 摘 要 】

Background

Polygoni Multiflori Radix, He-Shou-Wu in Chinese, is a widely used traditional Chinese medicine. Clinically, water decoction is the major application form of He-Shou-Wu. Therefore, simultaneous determination of bioactive compounds in water extract is very important for its quality control.

Results

A pressurized liquid extraction and short-end injection micellar electrokinetic chromatography (MEKC) were first developed for simultaneous determination of seven hydrophilic bioactive compounds in water extract of He-Shou-Wu. The influence of parameters, such as pH, concentration of phosphate, SDS and HP-β-CD, capillary temperature and applied voltage, on the analysis were carefully investigated. Optimum separation was obtained within 14 min by using 50 mM phosphate buffer containing 90 mM SDS and 2% (m/v) HP-β-CD (pH 2.5) at 15 kV and 20°C. All calibration curves showed good linearity (R²>0.9978) within test ranges. The overall LOD and LOQ were lower than 2.0 μg/mL and 5.5 μg/mL, respectively. The RSDs for intra- and inter-day of seven analytes were less than 3.2% and 4.6%, and the recoveries were 97.0%-104.2%.

Conclusion

The validated method was successfully applied to the analysis of He-Shou-Wu samples, which is helpful for its quality control.

【 授权许可】

   
2013 Lao et al; licensee Chemistry Central Ltd.

【 图 表 】

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