High throughput screening of hydrolytic enzymes from termites using a natural substrate derived from sugarcane bagasse

Biotechnology for Biofuels

Severino A Lucena²  Leile S Lima²  Luís SA Cordeiro²  Celso Sant'Anna²  Reginaldo Constantino¹  Patricia Azambuja³  Wanderley de Souza²  Eloi S Garcia³  Fernando A Genta³ 

[1] Zoology Department, University of Brasília, Campus Universitário Darcy Ribeiro - Instituto Central de Ciências Room AT-116, Brasília, 70910-900, Brazil

[2] Directory of Programs; National Institute of Metrology, Quality and Technology; Avenida Nossa Senhora das Graças, 50 - Xerém, Duque de Caxias, 25250-020, Brazil

[3] Department of Molecular Entomology, National Institute of Science and Technology, Avenida Brigadeiro Trompowsky, Centro de Ciências da Saúde, Building D-SS room 05, Rio de Janeiro, 21941-590, Brazil

关键词: termite;    sugarcane;    hydrolysis;    hemicellulase;    enzyme;    cellulase;    bagasse;   

Others  :  798378
DOI  :  10.1186/1754-6834-4-51

 received in 2011-09-06, accepted in 2011-11-14,  发布年份 2011

【 摘 要 】

Background

The description of new hydrolytic enzymes is an important step in the development of techniques which use lignocellulosic materials as a starting point for fuel production. Sugarcane bagasse, which is subjected to pre-treatment, hydrolysis and fermentation for the production of ethanol in several test refineries, is the most promising source of raw material for the production of second generation renewable fuels in Brazil. One problem when screening hydrolytic activities is that the activity against commercial substrates, such as carboxymethylcellulose, does not always correspond to the activity against the natural lignocellulosic material. Besides that, the macroscopic characteristics of the raw material, such as insolubility and heterogeneity, hinder its use for high throughput screenings.

Results

In this paper, we present the preparation of a colloidal suspension of particles obtained from sugarcane bagasse, with minimal chemical change in the lignocellulosic material, and demonstrate its use for high throughput assays of hydrolases using Brazilian termites as the screened organisms.

Conclusions

Important differences between the use of the natural substrate and commercial cellulase substrates, such as carboxymethylcellulose or crystalline cellulose, were observed. This suggests that wood feeding termites, in contrast to litter feeding termites, might not be the best source for enzymes that degrade sugarcane biomass.

【 授权许可】

   
2011 Lucena et al; licensee BioMed Central Ltd.

【 图 表 】

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