Carbon catabolite repression in Thermoanaerobacterium saccharolyticum

Biotechnology for Biofuels

Christopher D Herring¹  David A Hogsett¹  Bethany B Miller¹  A Joe Shaw¹  Vasiliki Tsakraklides¹ 

[1]Mascoma Corporation, 67 Etna Road, Suite 300, New Hampshire, 03766, Lebanon

关键词: Anaerobe;    Thermophile;    Ethanol;    Lignocellulose;    Deoxyglucose;    HPr;    Glucose;    Arabinose;    Catabolite repression;   

Others  :  798199
DOI  :  10.1186/1754-6834-5-85

 received in 2012-07-31, accepted in 2012-10-25,  发布年份 2012

【 摘 要 】

Background

The thermophilic anaerobe Thermoanaerobacterium saccharolyticum is capable of directly fermenting xylan and the biomass-derived sugars glucose, cellobiose, xylose, mannose, galactose and arabinose. It has been metabolically engineered and developed as a biocatalyst for the production of ethanol.

Results

We report the initial characterization of the carbon catabolite repression system in this organism. We find that sugar metabolism in T. saccharolyticum is regulated by histidine-containing protein HPr. We describe a mutation in HPr, His15Asp, that leads to derepression of less-favored carbon source utilization.

Conclusion

Co-utilization of sugars can be achieved by mutation of HPr in T. saccharolyticum. Further manipulation of CCR in this organism will be instrumental in achieving complete and rapid conversion of all available sugars to ethanol.

【 授权许可】

   
2012 Tsakraklides et al.; licensee BioMed Central Ltd.

【 图 表 】

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