A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes

Biomarker Research

Huiyuan Hu⁵  Yayoi Shikama⁴  Tsutomu Shichishima⁶  Kazuhiko Ikeda¹  Kazuko Akutsu²  Tomoyuki Ono⁴  Hideo Kimura³  Kazuei Ogawa¹  Hideyoshi Noji¹  Yasuchika Takeishi¹  Junko Kimura⁴ 

[1] Department of Cardiology and Hematology, Fukushima Medical University, Fukushima, Japan

[2] Department of Hematology, Iwaki Kyoritsu General Hospital, Iwaki, Japan

[3] Department of Hematology, Kita Fukushima Medical Center, Date, Japan

[4] Department of Pharmacology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan

[5] Department of Pharmaceutical Toxicology, China Medical University School of Pharmaceutical Sciences, Shenyang, China

[6] Fukushima Research Institute of Environment and Medicine, Futaba, Japan

关键词: Gene expression profile;    Fractionation;    Granulopoiesis;    C/EBP-ϵ;    AML1;    Myelodysplastic syndromes;   

Others  :  791793
DOI  :  10.1186/2050-7771-2-2

 received in 2013-12-27, accepted in 2014-01-06,  发布年份 2014

【 摘 要 】

We applied our new method, maturity-dependent fractionation of bone marrow-derived neutrophil progenitors, to a study of gene expression profiles during granulopoiesis in myelodysplastic syndromes. CD34⁺ cells with low density [F1], CD11b^-/CD16^- [F2], CD11b⁺/CD16^- [F3] and CD11b⁺/CD16^low [F4] with intermediate density, CD11b⁺/CD16^int [F5] and CD11b⁺/CD16^high [F6] with high density were isolated from six patients. Although AML1 and C/EBP-ϵ mRNA peaked at F1 and F4, respectively, in healthy individuals, C/EBP-ϵ was maximized at F2/F3 in all patients, two of whom showed simultaneous peaks of AML1 at F2. Thus, this fractionation is useful to detect mistimed induction of granulopoiesis-regulating genes in myelodysplastic syndromes.

【 授权许可】

   
2014 Hu et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

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