Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) I: transcriptome analysis of the effects of uniconazole on chlorophyll and endogenous hormone biosynthesis

Biotechnology for Biofuels

Yang Liu³  Yang Fang²  Mengjun Huang²  Yanling Jin²  Jiaolong Sun²  Xiang Tao²  Guohua Zhang²  Kaize He²  Yun Zhao¹  Hai Zhao² 

[1] Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China

[2] Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China

[3] Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu 610041, China

关键词: Pathway;    Photosynthetic rate;    Endogenous hormones;    Chlorophyll;    Uniconazole;   

Others  :  1177297
DOI  :  10.1186/s13068-015-0246-7

 received in 2014-10-31, accepted in 2015-03-24,  发布年份 2015

【 摘 要 】

Background

Duckweed is a novel aquatic bioenergy crop that is found ubiquitously throughout the world. Uniconazole plays an important role in improving crop production through the regulation of endogenous hormone levels. We found that a high quantity and quality of duckweed growth can be achieved by uniconazole application, although the mechanisms are unknown.

Results

The fronds of Landoltia punctata were sprayed evenly with 800 mg/L uniconazole. The dry weight following treatment increased by 10% compared to the controls at 240 h. Endogenous cytokinin (CK) and abscisic acid (ABA) content both increased compared to the control, while the level of gibberellins (GAs) decreased. Additionally, gene expression profiling results showed that the expression of transcripts encoding key enzymes involved in endogenous CK and ABA biosynthesis were up-regulated, while the transcripts of key enzymes for GAs biosynthesis were down-regulated. On the other hand, chlorophyll a and chlorophyll b contents were both increased compared with the control. Moreover, the net photosynthetic rate was elevated to 25.6 μmol CO₂/m²/s compared with the control value of 22.05 μmol CO₂/m²/s. Importantly, the expression of some chlorophyll biosynthesis-related transcripts was up-regulated.

Conclusion

Uniconazole treatment altered endogenous hormone levels and enhanced chlorophyll content and net photosynthetic rate in duckweed by regulating key enzymes involved in endogenous hormone and chlorophyll biosynthesis. The alterations of endogenous hormones and the increase of chlorophyll and photosynthetic rate data support the increase of biomass and starch accumulation.

【 授权许可】

   
2015 Liu et al.; licensee BioMed Central.

【 图 表 】

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