Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) II: transcriptome alterations of pathways involved in carbohydrate metabolism and endogenous hormone crosstalk

Biotechnology for Biofuels

Yang Liu²  Yang Fang²  Mengjun Huang²  Yanling Jin²  Jiaolong Sun²  Xiang Tao²  Guohua Zhang²  Kaize He²  Yun Zhao¹  Hai Zhao² 

[1] Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Sciences, Sichuan University, N0.24 South Section 1, Yihuan Road, Chengdu, 610064, China

[2] Environmental Microbiology Key Laboratory of Sichuan Province, No.9 Section 4, Renmin Nan Road, Chengdu, 610041, China

关键词: Pathway;    Crosstalk;    Uniconazole;    Endogenous hormones;    Starch accumulation;    Bioethanol;   

Others  :  1177290
DOI  :  10.1186/s13068-015-0245-8

 received in 2014-10-31, accepted in 2015-03-24,  发布年份 2015

【 摘 要 】

Background

Landoltia punctata is a widely distributed duckweed species with great potential to accumulate enormous amounts of starch for bioethanol production. We found that L. punctata can accumulate starch rapidly accompanied by alterations in endogenous hormone levels after uniconazole application, but the relationship between endogenous hormones and starch accumulation is still unclear.

Results

After spraying fronds with 800 mg/L uniconazole, L. punctata can accumulate starch quickly, with a dry weight starch content of up to 48% after 240 h of growth compared to 15.7% in the control group. Electron microscopy showed that the starch granule content was elevated after uniconazole application. The activities of key enzymes involved in starch synthesis were also significantly increased. Moreover, the expression of regulatory elements of the cytokinin (CK), abscisic acid (ABA) and gibberellin (GA) signaling pathways that are involved in chlorophyll and starch metabolism also changed correspondingly. Importantly, the expression levels of key enzymes involved in starch biosynthesis were up-regulated, while transcript-encoding enzymes involved in starch degradation and other carbohydrate metabolic branches were down-regulated.

Conclusion

The increase of endogenous ABA and CK levels positively promoted the activity of ADP-glucose pyrophosphorylase (AGPase) and chlorophyll content, while the decrease in endogenous GA levels inactivated α-amylase. Thus, the alterations of endogenous hormone levels resulted in starch accumulation due to regulation of the expression of genes involved in the starch metabolism pathway.

【 授权许可】

   
2015 Liu et al.; licensee BioMed Central.

【 图 表 】

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