Lung inflammation by fungus, Bjerkandera adusta isolated from Asian sand dust (ASD) aerosol and enhancement of ovalbumin-induced lung eosinophilia by ASD and the fungus in mice

Allergy, Asthma & Clinical Immunology

Boying Liu⁵  Takamichi Ichinose⁴  Miao He⁴  Fumihisa Kobayashi⁶  Teruya Maki⁶  Seiichi Yoshida⁴  Yasuhiro Yoshida⁷  Keiichi Arashidani⁷  Hirohisa Takano⁸  Masataka Nishikawa²  Guifan Sun¹  Takayuki Shibamoto³ 

[1] Environment and Chronic Non-communicable Disease Research Center, College of Public Health, China Medical University, 11001 Shenyang, China

[2] Environmental Chemistry Division, National Institute for Environmental Studies, 305-8506 Ibaraki, Japan

[3] Department of Environmental Toxicology, University of California, Davis, CA 95616, USA

[4] Department of Health Sciences, Oita University of Nursing and Health Sciences, 870-1201 Oita, Japan

[5] Present address: Department of Prophylaxis and Health Care, The Fourth Affiliated Hospital of China Medical University, 110032 Shenyang, China

[6] College of Science and Engineering, Kanazawa University, 920-1192 Ishikawa, Japan

[7] Department of Immunology and Parasitology, School of Medicine, University of Occupational and Environmental Health, Japan, 807-8555 Fukuoka, Japan

[8] Environmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, 615-8530 Kyoto, Japan

关键词: Asthma;    Lung eosinophilia;    Fungus;    Bjerkandera adusta;    Asian sand dust;   

Others  :  791925
DOI  :  10.1186/1710-1492-10-10

 received in 2013-11-16, accepted in 2014-01-30,  发布年份 2014

【 摘 要 】

Background

Bjerkandera adusta (B. adusta) is one of the most important etiological fungi associated with chronic cough. However, precise details of the inflammatory response to exposure are not well understood yet. B. adusta was recently identified in Asian sand dust (ASD) aerosol. Therefore, in the present study the exacerbating effects of ASD on B. adusta-induced lung inflammation and B. adusta + ASD on ovalbumin (OVA)-induced murine lung eosinophilia were investigated using experimental mice.

Methods

In order to prepare testing samples, B. adusta obtained from ASD aerosol was inactivated by formalin and ASD collected from the atmosphere was heated to remove toxic organic substances (H-ASD). CD-1 mice were instilled intratracheally with 12 different samples prepared with various combinations of B. adusta, H-ASD, and OVA in a normal saline solution. The lung pathology, cytological profiles in bronchoalveolar lavage fluid (BALF), and the levels of inflammatory cytokines/chemokines in BALF were investigated.

Results

H-ASD aggravated the lung eosinophilia induced by B. adusta alone, which also aggravated the lung eosinophilia induced by OVA. The mixture of OVA, H-ASD, and B. adusta caused serious fibrous thickening of the subepithelial layer, eosinophil infiltration, and proliferation of goblet cells in the airways along with remarkable increases of IL-13, eotaxin, IL-5, and MCP-3 in BALF.

Conclusions

The results of the present study demonstrated that B. adusta isolated from ASD aerosol induces allergic lung diseases. H-ASD enhanced allergic reactions caused by OVA or B. adusta. A mixture of B. adusta, H-ASD, and OVA caused the most remarkable exacerbation to the allergic airway inflammation via remarkable increases of pro-inflammatory mediators.

【 授权许可】

   
2014 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

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