Novel enzymes for the degradation of cellulose

Biotechnology for Biofuels

Vincent GH Eijsink¹  Bjørge Westereng¹  Gustav Vaaje-Kolstad¹  Svein Jarle Horn¹ 

[1]Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, Aas, Norway

关键词: Aldonic acid;    Bioeconomy;    Biorefinery;    Lytic polysaccharide monooxygenase;    Bioethanol;    Biofuel;    CBM33;    GH61;    Cellulose;    Cellulase;   

Others  :  798271
DOI  :  10.1186/1754-6834-5-45

 received in 2012-05-16, accepted in 2012-06-18,  发布年份 2012

【 摘 要 】

The bulk terrestrial biomass resource in a future bio-economy will be lignocellulosic biomass, which is recalcitrant and challenging to process. Enzymatic conversion of polysaccharides in the lignocellulosic biomass will be a key technology in future biorefineries and this technology is currently the subject of intensive research. We describe recent developments in enzyme technology for conversion of cellulose, the most abundant, homogeneous and recalcitrant polysaccharide in lignocellulosic biomass. In particular, we focus on a recently discovered new type of enzymes currently classified as CBM33 and GH61 that catalyze oxidative cleavage of polysaccharides. These enzymes promote the efficiency of classical hydrolytic enzymes (cellulases) by acting on the surfaces of the insoluble substrate, where they introduce chain breaks in the polysaccharide chains, without the need of first “extracting” these chains from their crystalline matrix.

【 授权许可】

   
2012 Horn et al.; licensee BioMed Central Ltd.

【 图 表 】

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