Optimization of ultrasonic extraction of polysaccharides from Ziziphus jujuba Mill. by response surface methodology

Chemistry Central Journal

Chenling Qu¹  Songcheng Yu³  Li Luo²  Yan Zhao¹  Yawei Huang¹ 

[1] College of Grain Oil and Food Science, Henan University of Technology, Zhengzhou 450052, China

[2] Changge Bureau of Quality and Technical Supervision, Changge 461500, China

[3] College of Public Health, Zhengzhou University, Zhengzhou 450001, China

关键词: Optimization;    Ziziphus jujuba Mill.;    Polysaccharides;    Response surface methodology (RSM);    Ultrasonic extraction;   

Others  :  787834
DOI  :  10.1186/1752-153X-7-160

 received in 2013-07-10, accepted in 2013-09-10,  发布年份 2013

【 摘 要 】

Background

Ziziphus jujuba Mill. is nutritious and used as food and medicine for more than two thousand years. It has many pharmacological effects, such as elimination of fatigue, dilation of blood vessels, etc. The polysaccharide in it is one of the bioactive substances. In this paper, the ultrasonic extraction effects on the yield and activity of polysaccharide were studied.

Results

The optimum ultrasonic extraction conditions were investigated based on a Box-Behnken statistical experimental design. Response surface methodology (RSM) of three factors (ultrasonic power, extraction time and extraction temperature) and three levels was employed to optimize the yield and the antioxidant activity of the polysaccharides. The experimental data were fitted to quadratic response surface models using multiple regression analysis. The best extraction conditions were 120 W, 15 min. and 55°C for highest yield, and 80 W, 15 min. and 40°C for highest hydroxyl radical scavenging activity.

Conclusion

The study showed that high ultrasonic power was good for obtaining high yield but bad for keeping the antioxidant activity of the polysaccharides.

【 授权许可】

   
2013 Qu et al.; licensee Chemistry Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Zhao Z, Liu M, Tu P: Characterization of water soluble polysaccharides from organs of Chinese Jujube (Ziziphus jujuba Mill. cv. Dongzao). Eur Food Res Technol 2008, 226:985-989.
• [2]Zhao Z, Dai H, Wu X, Chang H, Gao X, Liu M, Tu P: Characterization of a pectic polysaccharide from the fruit of Ziziphus jujuba. Chem Nat Compd 2007, 43(4):311-312.
• [3]Zhao Z, Li J, Wu X, Dai H, Gao X, Liu M, Tu P: Structures and immunological activities of two pectic polysaccharides from the fruits of Ziziphus jujuba Mill. cv. jinsixiaozao Hort. Food Res Int 2006, 39(8):917-923.
• [4]Li J, Shan L, Liu Y, Fan L, Ai L: Screening of a functional polysaccharide from Zizyphus Jujuba cv. Jinsixiaozao and its property. Int J Biol Macromol 2011, 49(3):255-259.
• [5]Chang SC, Hsu BY, Chen BH: Structural characterization of polysaccharides from Zizyphus jujuba and evaluation of antioxidant activity. Int J Biol Macromol 2010, 47(4):445-453.
• [6]Zhong K, Wang Q, He Y, He X: Evaluation of radicals scavenging, immunity-modulatory and antitumor activities of longan polysaccharides with ultrasonic extraction on in S180 tumor mice models. Int J Biol Macromol 2010, 47(3):356-360.
• [7]Lai F, Wen Q, Li L, Wu H, Li X: Antioxidant activities of water-soluble polysaccharide extracted from mung bean (Vigna radiata L.) hull with ultrasonic assisted treatment. Carbohyd Polym 2010, 81(2):323-329.
• [8]Pan Y, Dong S, Hao Y, Zhou Y, Ren X, Wang J, Wang W, Chu T: Ultrasonic-assisted extraction process of crude polysaccharides from Yunzhi mushroom and its effect on hydroxyproline and glycosaminoglycan levels. Carbohyd Polym 2010, 81(1):93-96.
• [9]Chen X, Tang Q, Chen Y, Wang W, Li S: Simultaneous extraction of polysaccharides from Poria cocos by ultrasonic technique and its inhibitory activities against oxidative injury in rats with cervical cancer. Carbohyd Polym 2010, 79(2):409-413.
• [10]Zhou C, Yu X, Zhang Y, He R, Ma H: Ultrasonic degradation, purification and analysis of structure and antioxidant activity of polysaccharide from Porphyra yezoensis Ueda. Carbohyd Polym 2012, 87(3):2046-2051.
• [11]Galai S, Touhami Y, Marzouki MN: Response surface methodology applied to laccases activities exhibited by stenotrophomonas maltophilia AAP56 in different growth conditions. Bioresources 2012, 7(1):706-726.
• [12]Liu J, Hu HR, Xu JF, Wen YB: Optimizing enzymatic pretreatment of recycled fiber to improve its draining ability using response surface methodology. Bioresources 2012, 7(2):2121-2140.
• [13]Xu P, Bao JS, Gao JJ, Zhou T, Wang YF: Optimization of extraction of phenolic antioxidants from tea (Camellia Sinensis L.) fruit peel biomass using response surface methodology. Bioresources 2012, 7(2):2431-2443.
• [14]Chen Y, Gu X, Huang S, Li J, Wang X, Tang J: Optimization of ultrasonic/microwave assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities. Int J Biol Macromol 2010, 46(4):429-435.
• [15]Zhong K, Wang Q: Optimization of ultrasonic extraction of polysaccharides from dried longan pulp using response surface methodology. Carbohyd Polym 2010, 80(1):10-25.
• [16]Huang S, Ning Z: Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity. Int J Biol Macromol 2010, 47(3):336-341.
• [17]Tian Y, Zeng H, Xu Z, Zheng B, Lin Y, Gan C, Lo YM: Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricus bisporus). Carbohyd Polym 2012, 88(2):522-529.
• [18]Zhang B, Yan P, Chen H, He J: Optimization of production conditions for mushroom polysaccharides with high yield and antitumor activity. Carbohyd Polym 2012, 87(4):2569-2575.
• [19]Chen W, Wang W, Zhang H, Huang Q: Optimization of ultrasonic-assisted extraction of water-soluble polysaccharides from Boletus edulis mycelia using response surface methodology. Carbohyd Polym 2011, 87(1):614-619.
• [20]Zhao Q, Kennedy JF, Wang X, Yuan X, Zhao B, Peng Y, Huang Y: Optimization of ultrasonic circulating extraction of polysaccharides from Asparagus officinalis using response surface methodology. Int J Biol Macromol 2011, 49(2):181-187.
• [21]Yang W, Fang Y, Liang J, Hu Q: Optimization of ultrasonic extraction of Flammulina velutipes polysaccharides and evaluation of its acetylcholinesterase inhibitory activity. Food Res Int 2011, 44(5):1269-1275.
• [22]Zou Y, Chen X, Yang W, Liu S: Response surface methodology for optimization of the ultrasonic extraction of polysaccharides from Codonopsis pilosula Nannf.var.modesta L.T. Shen. Carbohyd Polym 2011, 84(1):503-508.
• [23]Yan Y, Yu C, Chen J, Li X, Wang W, Li S: Ultrasonic-assisted extraction optimized by response surface methodology, chemical composition and antioxidant activity of polysaccharides from Tremella mesenterica. Carbohyd Polym 2011, 83(1):217-224.
• [24]Gan C, Latiff AA: Extraction of antioxidant pectic-polysaccharide from mangosteen (Garcinia mangostana) rind: Optimization using response surface methodology. Carbohyd Polym 2011, 83(2):600-607.
• [25]Ma L, Gan D, Wang M, Zhang Z, Jiang C, Zeng X: Optimization of extraction, preliminary characterization and hepatoprotective effects of polysaccharides from Stachys floridana Schuttl. ex Benth. Carbohyd Polym 2012, 87(2):1390-1398.
• [26]Pan WJ, Liao AM, Zhang JG, Dong Z, Wei ZJ: Supercritical carbon dioxide extraction of the oak silkworm (Antheraea pernyi) pupal oil: process optimization and composition determination. Int J Mol Sci 2012, 13(2):2354-2367.
• [27]Wei ZJ, Liao AM, Zhang HX, Liu J, Jiang ST: Optimization of supercritical carbon dioxide extraction of silkworm pupal oil applying the response surface methodology. Biores Technol 2009, 100(18):4214-4219.
• [28]Wei ZJ, Zhou LC, Chen H, Chen GH: Optimization of the fermentation conditions for 1-deoxynojirimycin production by streptomyces lawendulae applying the response surface methodology. Int J Food Eng 2011, 7(3):16. 12 pp